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详细说明
概述
产品名称Anti-EIF3S4抗体[EPR16146] - N-terminal
描述
兔单克隆抗体[EPR16146] to EIF3S4 - N-terminal
经测试应用WB,IHC-P,ICC/IF,Flow Cyt
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human EIF3S4 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: O75821阳性对照
293T, Molt-4, HepG2, K562, PC-12 and NIH/3T3 cell lysate. Human colonic carcinoma tissue. Rat skeletal muscle tissue. HepG2 cells. 293T cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS纯度Protein A purified
克隆单克隆
克隆编号EPR16146
同种型IgG
研究领域
Epigenetics and Nuclear Signaling
RNAi
Eukaryotic Initiation factors (eIF's)
Epigenetics and Nuclear Signaling
DNA / RNA
Translation
tRNAs
Anti-EIF3S4 antibody [EPR16146] - N-terminal 图像
![Western blot - Anti-EIF3S4 antibody [EPR16146] (ab191422)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab191422221.jpg)
Western blot - Anti-EIF3S4 antibody [EPR16146] (ab191422)
All lanes : Anti-EIF3S4 antibody [EPR16146] - N-terminal (ab191422) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Molt-4 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 36 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
![Western blot - Anti-EIF3S4 antibody [EPR16146] (ab191422)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab191422222.jpg)
Western blot - Anti-EIF3S4 antibody [EPR16146] (ab191422)
All lanes : Anti-EIF3S4 antibody [EPR16146] - N-terminal (ab191422) at 1/1000 dilution
Lane 1 : PC-12 cell lysate
Lane 2 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 36 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF3S4 antibody [EPR16146] (ab191422)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab191422223.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF3S4 antibody [EPR16146] (ab191422)
Immunohistochemical analysis of paraffin-embedded human colonic carcinoma tissue sections labeling EIF3S4 using ab191422 at a 1/50 dilution. A ready to use HRP polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF3S4 antibody [EPR16146] (ab191422)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab191422224.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF3S4 antibody [EPR16146] (ab191422)
Immunohistochemical analysis of paraffin embedded rat skeletal muscle tissue sections labeling EIF3S4 using ab191422 at a 1/50 dilution. A ready to use HRP polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.
![Immunocytochemistry/ Immunofluorescence - Anti-EIF3S4 antibody [EPR16146] (ab191422)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab191422225.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-EIF3S4 antibody [EPR16146] (ab191422)
Immunofluorescent analysis of 4% paraformaldehyde fixed HepG2 cells labeling EIF3S4 using ab191422 at a 1/150 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary at a 1/200 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100. The two negative controls: Primary ab concentration (anti-EIF3G), Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/400 dilution.
![Flow Cytometry - Anti-EIF3S4 antibody [EPR16146] (ab191422)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab191422226.jpg)
Flow Cytometry - Anti-EIF3S4 antibody [EPR16146] (ab191422)
Flow Cytometry analysis of 2% paraformaldehyde fixed 293T cells labeling EIF3S4 using ab191422 at a 1/80 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green).
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