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详细说明
概述
产品名称Anti-Emi1抗体[EPR15320-103]
描述
兔单克隆抗体[EPR15320-103] to Emi1
经测试应用WB,IHC-P,ICC/IF,IP
种属反应性
与反应: Mouse, Rat, Human
免疫原
Recombinant fragment within Human Emi1 aa 100-250. The exact sequence is proprietary.
Database link: Q9UKT4Run BLAST with
Run BLAST with 
阳性对照
HeLa, 293, HepG2 cell lysates; Human ovarian carcinoma and mouse liver tissues; HeLa and HepG2 cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR15320-103
同种型IgG
研究领域
Cell Biology
Proteolysis / Ubiquitin
Proteasome / Ubiquitin
Ubiquitin E3 Enzymes
F Box E3 Ligase
Anti-Emi1 antibody [EPR15320-103] 图像
![Western blot - Anti-Emi1 [EPR15320-103] antibody (ab187144)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab187144144.jpg)
Western blot - Anti-Emi1 [EPR15320-103] antibody (ab187144)
All lanes : Anti-Emi1 antibody [EPR15320-103] (ab187144) at 1/10000 dilution
Lane 1 : Hela cell lysate
Lane 2 : 293 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 50 kDa
Additional bands at : 56 kDa. We are unsure as to the identity of these extra bands.![Immunoprecipitation - Anti-Emi1 [EPR15320-103] antibody (ab187144)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab187144ddd.jpg)
Immunoprecipitation - Anti-Emi1 [EPR15320-103] antibody (ab187144)
Immunoprecipitation of Emi1 from HepG2 cell lysate using ab187144 at 1/50 (lane 1) or 1X PBS (negative control) (lane 2). Immunostained with ab187144 at 1/1000 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
![Immunocytochemistry/ Immunofluorescence - Anti-Emi1 [EPR15320-103] antibody (ab187144)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab187144rrr.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Emi1 [EPR15320-103] antibody (ab187144)
Immunofluorescent analysis of HepG2 cells labeling Emi1 with ab187144 at 1/250 and DAPI staining (blue).
![Immunocytochemistry/ Immunofluorescence - Anti-Emi1 [EPR15320-103] antibody (ab187144)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab187144eee.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Emi1 [EPR15320-103] antibody (ab187144)
Immunofluorescent analysis of HeLa cells labeling Emi1 with ab187144 at 1/100 and DAPI staining (blue).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emi1 [EPR15320-103] antibody (ab187144)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab187144IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emi1 [EPR15320-103] antibody (ab187144)
Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue labeling Emi1 with ab187144 at 1/100 with a prediluted HRP Polymer for Rabbit IgG as secondary antibody.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emi1 [EPR15320-103] antibody (ab187144)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab187144HCb.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emi1 [EPR15320-103] antibody (ab187144)
Immunohistochemical analysis of paraffin embedded Mouse liver tissue labeling Emi1 with ab187144 at 1/100 with a prediluted HRP Polymer for Rabbit IgG as secondary antibody.
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