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详细说明
概述
产品名称Anti-DDIT4抗体[EPR18716]
描述
兔单克隆抗体[EPR18716] to DDIT4
经测试应用IP,ICC/IF,WB
种属反应性
与反应: Human
免疫原
Recombinant full length protein within Human DDIT4 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q9NX09Run BLAST with
Run BLAST with 
阳性对照
WB: HeLa and MCF7 treated with 0.5 mM CoCl2 for 6 hours whole cell lysates. ICC/IF: HeLa cells treated with 0.5mM CoCl2 for 6 hours. IP: HeLa treated with 0.5mM CoCl2 for 6 hours whole cell lysate.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR18716
同种型IgG
研究领域
Metabolism
Pathways and Processes
Metabolism processes
Hypoxia
Cancer
Cancer Metabolism
Response to hypoxia
Epigenetics and Nuclear Signaling
Cardiovascular/Immune
Hypoxia
Hypoxia regulated
Cardiovascular
Hypoxia
Hypoxia-Regulated
Anti-DDIT4 antibody [EPR18716] 图像
![Western blot - Anti-DDIT4 antibody [EPR18716] (ab191871)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab1918711WB.jpg)
Western blot - Anti-DDIT4 antibody [EPR18716] (ab191871)
All lanes : Anti-DDIT4 antibody [EPR18716] (ab191871) at 1/1000 dilution
Lane 1 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with 0.5 mM CoCl2 for 6 hours whole cell lysate
Lane 3 : Untreated MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) treated with 0.5 mM CoCl2 for 6 hours whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size : 25 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
![Immunocytochemistry/ Immunofluorescence - Anti-DDIT4 antibody [EPR18716] (ab191871)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab1918711IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DDIT4 antibody [EPR18716] (ab191871)
Immunofluorescent analysis of 100 methanol-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, untreated or treated with CoCl2 (0.5 mM) for 6 hours, labeling DDIT4 with ab191871 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The expression increased after treatment with CoCl2 (0.5 mM) for 6 hours. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab191871 at 1/250 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.![Immunoprecipitation - Anti-DDIT4 antibody [EPR18716] (ab191871)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab191871P-1.jpg)
Immunoprecipitation - Anti-DDIT4 antibody [EPR18716] (ab191871)
DDIT4 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with 0.5mM CoCl2 for 6 hours whole cell lysate with ab191871 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab191871 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/10000 dilution.
Lane 1: HeLa treated with 0.5mM CoCl2 for 6h whole cell lysate, 10µg (Input).
Lane 2: ab191871 IP in HeLa treated with 0.5mM CoCl2 for 6h whole cell lysate.
Lane 3: Rabbit IgG,monoclonal- Isotype Control (ab172730) instead of ab191871 in HeLa treated with 0.5mM CoCl2 for 6h whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
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