详细说明
概述
产品名称Anti-DDX17抗体[EPR13807(B)] - C-terminal
描述
兔单克隆抗体[EPR13807(B)] to DDX17 - C-terminal
经测试应用ICC/IF,Flow Cyt,IHC-P,WB
种属反应性
与反应: Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DDX17 aa 100-200 (Cysteine residue). The exact sequence is proprietary.
Database link: Q92841阳性对照
293T, HeLa and MCF7 cell lysates; Human breast tissue; 293T cells; MCF7 cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 49% PBS, 0.05% BSA纯度Tissue culture supernatant
克隆单克隆
克隆编号EPR13807(B)
同种型IgG
研究领域
Epigenetics and Nuclear Signaling
DNA / RNA
RNA Processing
Other
Anti-DDX17 antibody [EPR13807(B)] - C-terminal 图像
Western blot - Anti-DDX17 [EPR13807(B)] antibody (ab180190)
All lanes : Anti-DDX17 antibody [EPR13807(B)] - C-terminal (ab180190) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF7 cell lysate
Lysates/proteins at 10 per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 72 kDa
Immunocytochemistry/ Immunofluorescence - Anti-DDX17 [EPR13807(B)] antibody (ab180190)
Immunofluorescent analysis of 293T cells labeling DDX17 with ab180190 at 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX17 [EPR13807(B)] antibody (ab180190)
Immunohistochemical analysis of paraffin embedded Human breast tissue labeling DDX17 with ab180190 at 1/100.
Flow Cytometry - Anti-DDX17 [EPR13807(B)] antibody (ab180190)
Flow Cytometrical analysis of permeabilized MCF7 cells labeling DDX17 with ab180190 at 1/10 (red) or a rabbit IgG negative (green).