详细说明
概述
产品名称Anti-DDX21抗体[EPR14495]
描述
兔单克隆抗体[EPR14495] to DDX21
经测试应用ICC/IF,IHC-P,WB
种属反应性
与反应: Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DDX21 aa 200-300. The exact sequence is proprietary.
Database link: Q9NR30阳性对照
HepG2, HEK293, Jurkat cell lysates. Human infiltrating duct carcinoma of breast tissue, HeLa cells and HepG2 cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Alternative versions available:
Anti-DDX21 antibody (Alexa Fluor® 647) [EPR14495] (ab203744)
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS纯度Tissue culture supernatant
克隆单克隆
克隆编号EPR14495
同种型IgG
研究领域
Epigenetics and Nuclear Signaling
Transcription
Other factors
Epigenetics and Nuclear Signaling
DNA / RNA
RNA Processing
Other
Anti-DDX21 antibody [EPR14495] 图像
Western blot - Anti-DDX21 [EPR14495] antibody (ab182156)
All lanes : Anti-DDX21 antibody [EPR14495] (ab182156) at 1/5000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HEK293 cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 87 kDa
Observed band size : 87 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX21 [EPR14495] antibody (ab182156)
Immunohistochemical analysis of paraffin-embedded Human infiltrating duct carcinoma of breast tissue, staining DDX21 with ab182156 at 1/100 dilution. Hematoxylin was used as a counterstain.
Immunocytochemistry/ Immunofluorescence - Anti-DDX21 [EPR14495] antibody (ab182156)
Immunofluorescence analysis of HeLa cells, staining DDX21 (red) with ab182156 at 1/100 dilution. An Alexa Fluor®555 goat anti rabbit IgG was used as a secondary antibody. Cells were fixed with -20℃ Acetone. Nuclei were counterstained with DAPI (blue).
Immunocytochemistry/ Immunofluorescence - Anti-DDX21 [EPR14495] antibody (ab182156)
Immunofluorescence analysis of HepG2 cells, staining DDX21 (green) with ab182156 at 1/100 dilution. An Alexa Fluor®488 goat anti rabbit IgG was used as a secondary antibody. Cells were fixed in 4% paraformaldehyde. Nuclei were counterstained with DAPI (blue).