概述

• 产品名称Anti-Desmoglein 2抗体[EPR6768]
     

• 描述

  兔单克隆抗体[EPR6768] to Desmoglein 2

• 经测试应用WB,IHC-P,Flow Cyt,ICC/IF

• 种属反应性

  与反应: Mouse, Rat, Human, Monkey    
   

• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (C terminal)

• 阳性对照    

• HepG2, HeLa, Human fetal heart, mouse heart and rat heart lysates; Human thyroid gland papillary carcinoma tissue; HepG2 cells

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液Preservative: 0.01% Sodium azide
  Constituents: 50% Glycerol, 0.05% BSA

• 纯度Tissue culture supernatant

• 克隆单克隆

• 克隆编号EPR6768

• 同种型IgG

• 研究领域

• Cardiovascular

• Heart

• Cardiac arrhythmias

• Cancer

• Invasion/microenvironment

• ECM

• Cell adhesion

• Cadherins

• <a javascript:void(0)>Signal Transduction

• Cytoskeleton / ECM

• Cell Adhesion

• Cadherins

Anti-Desmoglein 2 antibody [EPR6768] 图像

• 

  Flow Cytometry - Anti-Desmoglein 2 antibody [EPR6768] (ab150372)          

     Overlay histogram showing HeLa cells stained with ab150372 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab150372, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.  

• 

  Western blot - Anti-Desmoglein 2 antibody [EPR6768] (ab150372)          

     All lanes : Anti-Desmoglein 2 antibody [EPR6768] (ab150372) at 1/10000 dilution
  
  Lane 1 : HepG2 cell lysate
  Lane 2 : HeLa cell lysate
  Lane 3 : Human fetal heart lysate
  Lane 4 : mouse heart lysate
  Lane 5 : rat heart lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP labelled goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 122 kDa
   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2 antibody [EPR6768] (ab150372)          

     Immunohistochemical analysis of paraffin embedded Human thyroid gland papillary carcinoma tissue labeling Desmoglein 2 with ab150372 at a 1/250 dilution.  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Desmoglein 2 antibody [EPR6768] (ab150372)          

     Immunofluorescent staining of HepG2 cells labeling Desmoglein 2 with ab150372 at a 1/250 dilution.