概述

• 产品名称Anti-DUSP5抗体[EPR19684]
     

• 描述

  兔单克隆抗体[EPR19684] to DUSP5

• 经测试应用WB

• 种属反应性

  与反应: Mouse, Human    
   

• 免疫原

  Recombinant full length protein within Human DUSP5 aa 1 to the C-terminus. The exact sequence is proprietary.
  Database link: Q16690

       Run BLAST with      Run BLAST with    

• 阳性对照    

• WB: Human DUSP5 full length recombinant protein; Mouse DUSP5 full length recombinant protein; MEF (WT) whole cell lysate.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam's RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol, 0.05% BSA

• 
  

• 纯度Protein A purified

• 克隆单克隆

• 克隆编号EPR19684

• 同种型IgG

• 研究领域

• Signal Transduction

• Protein Phosphorylation

• Tyrosine Phosphatases

Anti-DUSP5 antibody [EPR19684] 图像

• 

  Western blot - Anti-DUSP5 antibody [EPR19684] (ab200708)          

     All lanes : Anti-DUSP5 antibody [EPR19684] (ab200708) at 1/1000 dilution
  
  Lane 1 : MEF (Mouse embryonic fibroblast cell line) (WT) whole cell lysate
  Lane 2 : MEF (Mouse embryonic fibroblast cell line) (DUSP5 KO) whole cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
  
  Predicted band size : 42 kDa
  Observed band size : 42 kDa
  
  
  Exposure time : 3 minutes    

  Blocking/Dilution buffer: 5% NFDM/TBST.

  Lysates were kindly provided by Steve Keyes from Dundee University.

   

• 

  Western blot - Anti-DUSP5 antibody [EPR19684] (ab200708)          

     Anti-DUSP5 antibody [EPR19684] (ab200708) at 1/5000 dilution + Human DUSP5 full length recombinant protein at 0.01 µg
  
  Secondary
  Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
  
  Predicted band size : 42 kDa
  
  
  Exposure time : 8 seconds    

  Blocking/Dilution buffer: 5% NFDM/TBST.

  Human DUSP5 full length recombinant protein contain aa1-384 with a His-Tag®. This recombinant human full length protein was made in-house.

   

• 

  Western blot - Anti-DUSP5 antibody [EPR19684] (ab200708)          

     Anti-DUSP5 antibody [EPR19684] (ab200708) at 1/1000 dilution + Mouse DUSP5 full length recombinant protein at 0.01 µg
  
  Secondary
  Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
  
  Predicted band size : 42 kDa
  
  
  Exposure time : 4 seconds    

  Blocking/Dilution buffer: 5% NFDM/TBST.

  Mouse DUSP5 full length recombinant protein contain aa1-384 with a His-Tag®. This recombinant mouse full length protein was made in-house.