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详细说明
概述
产品名称Anti-DUSP9抗体[EPR15541]
描述
兔单克隆抗体[EPR15541] to DUSP9
经测试应用WB,ICC/IF
种属反应性
与反应: Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DUSP9 aa 300 to the C-terminus. The exact sequence is proprietary.
Database link: Q99956阳性对照
HeLa and 293 cell lysates; HepG2 cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR15541
同种型IgG
研究领域
Signal Transduction
Protein Phosphorylation
Ser / Thr Phosphatases
Anti-DUSP9 antibody [EPR15541] 图像
![Western blot - Anti-DUSP9 antibody [EPR15541] (ab194355)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab194355b-1.jpg)
Western blot - Anti-DUSP9 antibody [EPR15541] (ab194355)
All lanes : Anti-DUSP9 antibody [EPR15541] (ab194355) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 42 kDa
Observed band size : 42 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
![Immunocytochemistry/ Immunofluorescence - Anti-DUSP9 antibody [EPR15541] (ab194355)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab1943555IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DUSP9 antibody [EPR15541] (ab194355)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 cells labeling DUSP9 with ab194355 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution. The nuclear counter stain is DAPI (blue).
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