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详细说明
概述
产品名称Anti-Dynein intermediate chain 1抗体[EPR11244-61]
描述
兔单克隆抗体[EPR11244-61] to Dynein intermediate chain 1
经测试应用WB,ICC/IF
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Dynein intermediate chain 1.
Database link: Q9UI46阳性对照
Human, rat and mouse testis lysates; Human testis and trachea tissues; Jurkat cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS纯度Protein A purified
克隆单克隆
克隆编号EPR11244-61
同种型IgG
研究领域
Neuroscience
Cell Type Marker
Neuron marker
Axon marker
Signal Transduction
Cytoskeleton / ECM
Cytoskeleton
Motor Proteins
Dynein
Neuroscience
Cell Adhesion Proteins
Cytoskeletal Proteins
Microtubules
Anti-Dynein intermediate chain 1 antibody [EPR11244-61] 图像
![Western blot - Anti-Dynein intermediate chain 1 antibody [EPR11244-61] (ab171964)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab171964B-1.jpg)
Western blot - Anti-Dynein intermediate chain 1 antibody [EPR11244-61] (ab171964)
Anti-Dynein intermediate chain 1 antibody [EPR11244-61] (ab171964) at 1/5000 dilution (purified) + Human testis tissue lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size : 79 kDa
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
![Western blot - Anti-Dynein intermediate chain 1 antibody [EPR11244-61] (ab171964)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab171964B-2.jpg)
Western blot - Anti-Dynein intermediate chain 1 antibody [EPR11244-61] (ab171964)
Anti-Dynein intermediate chain 1 antibody [EPR11244-61] (ab171964) at 1/1000 dilution (purified) + Rat testis lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size : 79 kDa
Observed band size : 79 kDa
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
![Western blot - Anti-Dynein intermediate chain 1 antibody [EPR11244-61] (ab171964)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab171964B-3.jpg)
Western blot - Anti-Dynein intermediate chain 1 antibody [EPR11244-61] (ab171964)
Anti-Dynein intermediate chain 1 antibody [EPR11244-61] (ab171964) at 1/5000 dilution (purified) + Mouse testis tissue lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size : 79 kDa
Observed band size : 79 kDa
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
![Immunocytochemistry/ Immunofluorescence - Anti-Dynein intermediate chain 1 antibody [EPR11244-61] (ab171964)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab171964ICC.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Dynein intermediate chain 1 antibody [EPR11244-61] (ab171964)
Immunofluorescence staining of F9 cells with purified ab171964 at a working dilution of 1 in 250, counter-stained with DAPI and mouse monoclonal anti-tubulin (ab7291, 1/500). The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom right hand and middle panels - for the negative control, purified ab171964 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500.
![Western blot - Anti-Dynein intermediate chain 1 antibody [EPR11244-61] (ab171964)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab171964WB1.jpg)
Western blot - Anti-Dynein intermediate chain 1 antibody [EPR11244-61] (ab171964)
All lanes : Anti-Dynein intermediate chain 1 antibody [EPR11244-61] (ab171964) at 1/1000 dilution (unpurified)
Lane 1 : Human testis lysate
Lane 2 : rat testis lysate
Lane 3 : mouse testis lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
developed using the ECL technique
Predicted band size : 79 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Dynein intermediate chain 1 antibody [EPR11244-61] (ab171964)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab171964IF1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Dynein intermediate chain 1 antibody [EPR11244-61] (ab171964)
Immunofluorescence analysis of Jurkat cells, labeling Dynein intermediate chain 1 using unpurified ab171964 at a 1/50 dilution (green) and DAPI nuclear staining (blue).
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