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详细说明
概述
产品名称Anti-Firefly Luciferase抗体[EPR17789] - N-terminal
描述
兔单克隆抗体[EPR17789] to Firefly Luciferase - N-terminal
经测试应用ICC/IF,WB
种属反应性
与反应: Firefly (Photinus pyralis)
不与反应: Mouse, Rat, Human免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Firefly (Photinus pyralis) Firefly Luciferase aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P08659阳性对照
WB: Firefly Luciferase transfected HEK293 whole cell lysate. ICC/IF: Firefly Luciferase transfected HEK293T cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR17789
同种型IgG
研究领域
Tags & Cell Markers
Fusion / Marker Proteins
Luciferase
Anti-Firefly Luciferase antibody [EPR17789] - N-terminal 图像
![Western blot - Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab185923-wb.jpg)
Western blot - Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)
All lanes : Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923) at 1/2000 dilution
Lane 1 : Empty vector (vector control) transfected HEK293 (human embryonic kidney) whole cell lysate
Lane 2 : Firefly Luciferase transfected HEK293 (human embryonic kidney) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size : 61 kDa
Observed band size : 61 kDa
Exposure time : 30 secondsBlocking and dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab185923wb2.jpg)
Western blot - Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)
All lanes : Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923) at 1/1000 dilution
Lane 1 : Firefly Luciferase transfected HEK293 (human embryonic kidney) whole cell lysate at 10 µg
Lane 2 : HeLa (human cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 3 : MCF-7 (human breast carcinoma) whole cell lysate at 20 µg
Lane 4 : Jurkat (human acute T cell leukemia) whole cell lysate at 20 µg
Lane 5 : SH-SY5Y (human neuroblastoma) whole cell lysate at 20 µg
Lane 6 : Human fetal heart lysate at 10 µg
Lane 7 : Human fetal kidney lysate at 10 µg
Lane 8 : Human fetal spleen lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size : 61 kDa
Observed band size : 61 kDa
Exposure time : 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab185923wb3.jpg)
Western blot - Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)
All lanes : Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923) at 1/1000 dilution
Lane 1 : Firefly Luciferase transfected HEK293 (human embryonic kidney) whole cell lysate
Lane 2 : Mouse brain lysate
Lane 3 : Mouse heart lysate
Lane 4 : Mouse kidney lysate
Lane 5 : Mouse spleen lysate
Lane 6 : Rat brain lysate
Lane 7 : Rat kidney lysate
Lane 8 : Rat spleen lysate
Lane 9 : C6 (rat glioma) whole cell lysate lysate
Lane 10 : Raw264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate
Lane 11 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lane 12 : NIH/3T3 (mouse embryo) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size : 61 kDa
Observed band size : 61 kDa
Exposure time : 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
![Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1859231-c.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK293T (Human epithelial cells from embryonic kidney) cells transfected with Empty vector or Firefly Luciferase, labeling Firefly Luciferase with ab185923 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on HEK293T cells transfected with Firefly Luciferase is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
-ve control 1: ab185923 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
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