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详细说明
概述
产品名称Anti-Caveolin-1抗体[EPR15554] - N-terminal
描述
兔单克隆抗体[EPR15554] to Caveolin-1 - N-terminal
经测试应用WB,IHC-P,ICC/IF,Flow Cyt,IP
种属反应性
与反应: Mouse, Human
免疫原
Recombinant fragment within Human Caveolin-1 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q03135Run BLAST with
Run BLAST with 
阳性对照
PC-3, A549, A431 cell lysates; Human liver and squamous cell carcinoma of cervix tissues; mouse lung tissue; A673 cells; NIH3T3 cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR15554
同种型IgG
研究领域
Metabolism
Pathways and Processes
Metabolism processes
Hypoxia
Metabolism
Pathways and Processes
Metabolic signaling pathways
Lipid and lipoprotein metabolism
Cholesterol Metabolism
Cancer
Cancer Metabolism
Response to hypoxia
Cancer
Tumor biomarkers
Other
Tags & Cell Markers
Subcellular Markers
Organelles
Caveolae and Clathrin
Signal Transduction
Protein Trafficking
Vesicle Transport
Coat Proteins
Cardiovascular
Lipids / Lipoproteins
Lipid Metabolism
Cholesterol Metabolism
Anti-Caveolin-1 antibody [EPR15554] - N-terminal 图像
![Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody [EPR15554] (ab192869)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab192869-if.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody [EPR15554] (ab192869)
Immunofluorescence analysis of A673 cells labeling Caveolin-1 using ab192869 at 1/300 dilution (5 μg/ml). A Goat anti rabbit IgG (Alexa Fluor®488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permealized with 0.1% triton X-100. Counterstain: DAPI.
![Western blot - Anti-Caveolin-1 antibody [EPR15554] (ab192869)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab192869b-1.jpg)
Western blot - Anti-Caveolin-1 antibody [EPR15554] (ab192869)
All lanes : Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869) at 1/10000 dilution
Lane 1 : PC-3 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 17, 20 kDa
![Immunoprecipitation - Anti-Caveolin-1 antibody [EPR15554] (ab192869)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab192869-ip.jpg)
Immunoprecipitation - Anti-Caveolin-1 antibody [EPR15554] (ab192869)
Immunoprecipitation analysis of A431 cell lysate labeling Caveolin-1 using ab192869 at 1/30 dilution (Lane 1). PBS negative control (Lane 2). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as the secondary antibody.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [EPR15554] (ab192869)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab192869c-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [EPR15554] (ab192869)
Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix tissue labeling Caveolin-1 using ab192869 at 1/4000 dilution (0.15 μg/ml). A prediluted HRP Polymer for Rabbit IgG was used as the secondary. Counterstain: Hematoxylin. Negative control also shown.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [EPR15554] (ab192869)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab192869c-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [EPR15554] (ab192869)
Immunohistochemical analysis of paraffin-embeddedMouse lung tissue labeling Caveolin-1 using ab192869 at 1/4000 dilution (0.15 μg/ml). A prediluted HRP Polymer for Rabbit IgG was used as the secondary. Counterstain: Hematoxylin. Negative control also shown.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [EPR15554] (ab192869)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab192869c-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [EPR15554] (ab192869)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Caveolin-1 using ab192869 at 1/4000 dilution (0.15 μg/ml). A prediluted HRP Polymer for Rabbit IgG was used as the secondary. Counterstain: Hematoxylin. Negative control also shown.
![Flow Cytometry - Anti-Caveolin-1 antibody [EPR15554] (ab192869)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab192869-fc.jpg)
Flow Cytometry - Anti-Caveolin-1 antibody [EPR15554] (ab192869)
Flow cytometry analysis of NIH3T3 cells labeling Caveolin-1 using ab192869 at a 1/120 dilution (Red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Cells without incubation with primary antibody and secondary antibody Blue. Rabbit monoclonal IgG was used as isotype control (Black).
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