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详细说明
概述
产品名称Anti-gamma Sarcoglycan抗体[EPR17862-7]
描述
兔单克隆抗体[EPR17862-7] to gamma Sarcoglycan
经测试应用IHC-P,WB,IP
种属反应性
与反应: Human
免疫原
Recombinant fragment within Human gamma Sarcoglycan aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: Q13326Run BLAST with
Run BLAST with 
阳性对照
WB: Human skeletal muscle, MCF-7, Human fetal brain, Human fetal heart, Human fetal kidney, Human fetal spleen, Mouse muscle or Mouse heart whole cell lysate. IHC-P: Human skeletal muscle. IP: Human fetal kidney whole cell lysate
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR17862-7
同种型IgG
研究领域
Signal Transduction
Cytoskeleton / ECM
Extracellular Matrix
ECM Proteins
Other ECM Proteins
Anti-gamma Sarcoglycan antibody [EPR17862-7] 图像
![Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab203113WB1.jpg)
Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)
Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113) at 1/10000 dilution + Human skeletal muscle whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 32 kDa
Observed band size : 32 kDa
Exposure time : 15 secondsBlocking/Dilution buffer
5% NFDM/TBST.
This protein is expressed in skeletal and heart muscle.
![Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab203113WB2.jpg)
Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)
Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113) at 1/2000 dilution + MCF-7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 32 kDa
Observed band size : 32 kDa
Exposure time : 3 minutesBlocking/dilution buffer
5% NFDM/TBST.
![Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab203113WB3.jpg)
Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)
All lanes : Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113) at 1/2000 dilution
Lane 1 : Human fetal brain whole cell lysate
Lane 2 : Human fetal heart whole cell lysate
Lane 3 : Human fetal kidney whole cell lysate
Lane 4 : Human fetal spleen whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 32 kDa
Observed band size : 32 kDa
Exposure time : 15 secondsBlocking/dilution buffer 5% NFDM/TBST.
This protein is expressed in skeletal and heart muscle.
ab181602 Anti-GAPDH antibody [EPR16891] control used.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab203113HC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling gamma Sarcoglycan with ab203113 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a 1/500 dilution. Cell membrane and weak cytoplasmic staining observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, with secondary ab97051 Goat Anti-Rabbit IgG H&L (HRP).
![Immunoprecipitation - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab203113113.jpg)
Immunoprecipitation - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)
Human gamma Sarcoglycan was immunoprecipitated from 1mg of Human fetal kidney whole cell lysate with ab203113 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203113 at 1/10000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Human fetal kidney whole cell lysate 10 µg (Input). Lane 2: ab203113 IP in Human fetal kidney whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203113 in Human fetal kidney whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 SecondsBlocking/Dilution buffer 5% NFDM/TBST.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab203113HC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling gamma Sarcoglycan with ab203113 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a 1/500 dilution. No staining is observed on Human colon tissue. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, with secondary ab97051 Goat Anti-Rabbit IgG H&L (HRP).
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