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详细说明
概述
产品名称Anti-GBF1抗体[EPR14889] - C-terminal
描述
兔单克隆抗体[EPR14889] to GBF1 - C-terminal
经测试应用ICC/IF,Flow Cyt,WB,IHC-P
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human GBF1 aa 1800 to the C-terminus. The exact sequence is proprietary.
Database link: Q92538阳性对照
HeLa, Jurkat, 293, PC12 and NIH 3T3 cell lysates; Human kidney and Mouse cardiac muscle tissues; 293 and HeLa cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR14889
同种型IgG
研究领域
Signal Transduction
Protein Trafficking
Golgi Proteins
Signal Transduction
Protein Trafficking
Vesicle Transport
Coat Proteins
Signal Transduction
Protein Trafficking
ER Proteins
Signal Transduction
Protein Trafficking
Vesicle Transport
Regulation
Anti-GBF1 antibody [EPR14889] - C-terminal 图像
![Western blot - Anti-GBF1 [EPR14889] antibody - C-terminal (ab189512)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab189512WBa.jpg)
Western blot - Anti-GBF1 [EPR14889] antibody - C-terminal (ab189512)
All lanes : Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512) at 1/20000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size : 206 kDa
Observed band size : 250 kDa (why is the actual band size different from the predicted?)
![Western blot - Anti-GBF1 [EPR14889] antibody - C-terminal (ab189512)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab189512WBb.jpg)
Western blot - Anti-GBF1 [EPR14889] antibody - C-terminal (ab189512)
Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512) at 1/50000 dilution + 293 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size : 206 kDa
Observed band size : 250 kDa (why is the actual band size different from the predicted?)
![Western blot - Anti-GBF1 [EPR14889] antibody - C-terminal (ab189512)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab189512WBc.jpg)
Western blot - Anti-GBF1 [EPR14889] antibody - C-terminal (ab189512)
All lanes : Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512) at 1/5000 dilution
Lane 1 : PC12 cell lysate
Lane 2 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size : 206 kDa
Observed band size : 250 kDa (why is the actual band size different from the predicted?)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBF1 [EPR14889] antibody - C-terminal (ab189512)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab189512HCa.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBF1 [EPR14889] antibody - C-terminal (ab189512)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling GBF1 with ab189512 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin (Inset: negative control).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBF1 [EPR14889] antibody - C-terminal (ab189512)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab189512HCb.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBF1 [EPR14889] antibody - C-terminal (ab189512)
Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling GBF1 with ab189512 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin (Inset: negative control).
![Immunocytochemistry/ Immunofluorescence - Anti-GBF1 [EPR14889] antibody - C-terminal (ab189512)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1895122IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GBF1 [EPR14889] antibody - C-terminal (ab189512)
Immunofluorescent analysis of 4% paraformaldehyde-fixed 293 cells labeling GBF1 with ab189512 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with DAPI (blue).
![Flow Cytometry - Anti-GBF1 [EPR14889] antibody - C-terminal (ab189512)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1895122FC.jpg)
Flow Cytometry - Anti-GBF1 [EPR14889] antibody - C-terminal (ab189512)
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling GBF1 with ab189512 at 1/230 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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