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详细说明
概述
产品名称Anti-GCAT抗体[EPR13450] - N-terminal
描述
兔单克隆抗体[EPR13450] to GCAT - N-terminal
经测试应用WB,ICC/IF,IP,IHC-P
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human GCAT aa 1-150 (N terminal). The exact sequence is proprietary.
Database link: O75600阳性对照
BxPC-3, HepG2 and U87-MG cell lysates. Human fetal liver tissue lysate. Human heart and pancreas tissue. HepG2 cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 59% PBS纯度Tissue culture supernatant
克隆单克隆
克隆编号EPR13450
同种型IgG
研究领域
Metabolism
Pathways and Processes
Metabolic signaling pathways
Amino acid metabolism
Signal Transduction
Metabolism
Amino Acids
Anti-GCAT antibody [EPR13450] - N-terminal 图像
![Western blot - Anti-GCAT [EPR13450] antibody - N-terminal (ab181094)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181094WB1.jpg)
Western blot - Anti-GCAT [EPR13450] antibody - N-terminal (ab181094)
All lanes : Anti-GCAT antibody [EPR13450] - N-terminal (ab181094) at 1/5000 dilution
Lane 1 : BxPC-3 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : U87-MG cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 45 kDa
Observed band size : 45 kDa
![Western blot - Anti-GCAT [EPR13450] antibody - N-terminal (ab181094)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181094WB2.jpg)
Western blot - Anti-GCAT [EPR13450] antibody - N-terminal (ab181094)
Anti-GCAT antibody [EPR13450] - N-terminal (ab181094) at 1/5000 dilution + Human fetal liver lysate
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size : 45 kDa
Observed band size : 45 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCAT [EPR13450] antibody - N-terminal (ab181094)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181094HC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCAT [EPR13450] antibody - N-terminal (ab181094)
Immunohistochemical analysis of Human heart tissue, staining GCAT with ab181094 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCAT [EPR13450] antibody - N-terminal (ab181094)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181094HC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCAT [EPR13450] antibody - N-terminal (ab181094)
Immunohistochemical analysis of Human pancreas tissue, staining GCAT with ab181094 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.
![Immunocytochemistry/ Immunofluorescence - Anti-GCAT [EPR13450] antibody - N-terminal (ab181094)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1810944IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GCAT [EPR13450] antibody - N-terminal (ab181094)
Immunofluorescence analysis of HepG2 cells, staining GCAT (green) with ab181094 at 1/100 dilution. An Alexa Fluor®488-conjugated goat anti-rabbit IgG was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).
![Immunoprecipitation - Anti-GCAT [EPR13450] antibody - N-terminal (ab181094)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1810944IP.jpg)
Immunoprecipitation - Anti-GCAT [EPR13450] antibody - N-terminal (ab181094)
Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate (lane 1) or negative control (lane 2), labeling GCAT immunoprecipitated using ab181094 at 1/40 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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