概述

• 产品名称Anti-Caspase-8抗体[E7]
     

• 描述

  兔单克隆抗体[E7] to Caspase-8

• 特异性The antibody should recognize both pro-form (55kDa) and p18 cleaved-form of Caspase-8.

• 经测试应用WB,ICC/IF,Flow Cyt

• 种属反应性

  与反应: Human    
   

• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caspase-8 aa 200-300. A synthetic peptide corresponding to N-terminal residues of human Caspase-8 subunit p18 was used as immunogen.
  Database link: Q14790

• 阳性对照    

• Hela cell lysate

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated "PUR" on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

  Alternative versions available:

  Anti-Caspase-8 antibody (HRP) [E7] (ab194145)

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液pH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol, 0.05% BSA

• 
  

• 纯度Protein A purified

• 克隆单克隆

• 克隆编号E7

• 同种型IgG

• 研究领域

• Cancer

• Cell Death

• Apoptosis

• Metabolism

• Cancer

• Cell Death

• Necroptosis

• Cancer

• Cell Death

• Apoptosis

• Apoptotic Markers

• Caspases

• Metabolism

• Pathways and Processes

• Metabolism processes

• Apoptosis

• Cell Biology

• Proteolysis / Ubiquitin

• Proteolytic enzymes

• Other proteases

• Cancer

• Invasion/microenvironment

• Apoptosis

• Caspases

• Cell Biology

• Apoptosis

• Intracellular

• Caspases etc

• Caspases

Anti-Caspase-8 antibody [E7] 图像

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Caspase-8 antibody [E7] (ab32397)          

     

  ab32394 staining Caspase-8 in the K562 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/150). ab150078(1/500) an anti-rabbit Alexa Fluor^®555 was used as the secondary antibody. Nuclei were counterstained with DAPI.

   

• 

  Western blot - Anti-Caspase-8 antibody [E7] (ab32397)          

     All lanes : Anti-Caspase-8 antibody [E7] (ab32397) at 1/1000 dilution
  
  Lane 1 : Jurkat cell lysate
  Lane 2 : HeLa cell lysate
  Lane 3 : IM-9 cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution
  
  Predicted band size : 55 kDa
     

  Blocking buffer and concentration: 5% NFDM/TBST.

  Diluting buffer and concentration: 5% NFDM /TBST.

   

• 

  Western blot - Anti-Caspase-8 [E7] antibody (ab32397)          

     Anti-Caspase-8 antibody [E7] (ab32397) at 1/500 dilution (unpurified) + 10ug HeLa cell lysate.
  
  Predicted band size : 55 kDa
  Observed band size : 55 kDa