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详细说明
概述
产品名称Anti-Caspase-8抗体[EPR162]
描述
兔单克隆抗体[EPR162] to Caspase-8
经测试应用WB,IHC-P,ICC/IF
种属反应性
与反应: Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caspase-8 aa 200-300 (internal sequence).
Database link: Q14790阳性对照
Jurkat, IM9, HepG2, HeLa, and HL60 cell lysates; Human tonsil tissue and Human hepatocellular carcinoma tissue; HeLa cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Alternative versions available:
Anti-Caspase-8 antibody (Alexa Fluor® 488) [EPR162] (ab206068)
性能
形式Liquid
存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
纯度Tissue culture supernatant
克隆单克隆
克隆编号EPR162
同种型IgG
研究领域
Cancer
Cell Death
Apoptosis
Metabolism
Cancer
Cell Death
Necroptosis
Cancer
Cell Death
Apoptosis
Apoptotic Markers
Caspases
Metabolism
Pathways and Processes
Metabolism processes
Apoptosis
Cell Biology
Proteolysis / Ubiquitin
Proteolytic enzymes
Other proteases
Cancer
Invasion/microenvironment
Apoptosis
Caspases
Cell Biology
Apoptosis
Intracellular
Caspases etc
Caspases
Anti-Caspase-8 antibody [EPR162] 图像
![Western blot - Anti-Caspase-8 antibody [EPR162] (ab108333)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab10/ab108333OWt.jpg)
Western blot - Anti-Caspase-8 antibody [EPR162] (ab108333)
Predicted band size : 55 kDa
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Caspase-8 knockout HAP1 cell lysate (20 µg)
Lanes 1 and 2: Merged signal (red and green). Green - ab108333 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab108333 was shown to specifically react with Caspase-8 when Caspase-8 knockout samples were used. Wild-type and Caspase-8 knockout samples were subjected to SDS-PAGE. ab108333 and ab8245 (loading control to GAPDH) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.![Western blot - Anti-Caspase-8 [EPR162] antibody (ab108333)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab10/ab1083333-8.jpg)
Western blot - Anti-Caspase-8 [EPR162] antibody (ab108333)
All lanes : Anti-Caspase-8 antibody [EPR162] (ab108333) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : IM9 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HL60 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size : 55 kDa
![Western blot - Anti-Caspase-8 [EPR162] antibody (ab108333)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab10/ab1083333-2.jpg)
Western blot - Anti-Caspase-8 [EPR162] antibody (ab108333)
All lanes : Anti-Caspase-8 antibody [EPR162] (ab108333) at 1/1000 dilution
Lane 1 : Jurkat cell lysate, treated with etoposide
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate, treated staurosporine
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size : 55 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-8 [EPR162] antibody (ab108333)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab10/ab1083333-3.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-8 [EPR162] antibody (ab108333)
ab108333, at 1/100 dilution, staining Caspase-8 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-8 [EPR162] antibody (ab108333)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab10/ab1083333-4.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-8 [EPR162] antibody (ab108333)
ab108333, at 1/100 dilution, staining Caspase-8 in paraffin-embedded Human hepatocellular carcinoma by Immunohistochemistry.
![Immunocytochemistry/ Immunofluorescence - Anti-Caspase-8 [EPR162] antibody (ab108333)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab10/ab1083333-7.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Caspase-8 [EPR162] antibody (ab108333)
ab108333, at 1/100 dilution, staining Caspase-8 in HeLa cells by Immunofluorescence.
![Immunocytochemistry/ Immunofluorescence - Anti-Caspase-8 [EPR162] antibody (ab108333)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab10/ab108333-11.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Caspase-8 [EPR162] antibody (ab108333) Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
ab108333 (1/200) staining Caspase-8 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton-X100 (in PBS) and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to abreview.
See Abreview
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