详细说明
概述
产品名称Anti-CINP抗体[EPR14445] - N-terminal
描述
兔单克隆抗体[EPR14445] to CINP - N-terminal
经测试应用WB,ICC/IF,IP
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CINP aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q9BW66阳性对照
293, Jurkat, and HeLa lysate. HeLa cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR14445
同种型IgG
研究领域
Cell Biology
Cell Cycle
Kinases/Phosphatases
Cdk Regulators
Anti-CINP antibody [EPR14445] - N-terminal 图像
Western blot - Anti-CINP [EPR14445] antibody - N-terminal (ab183519)
All lanes : Anti-CINP antibody [EPR14445] - N-terminal (ab183519) at 1/1000 dilution
Lane 1 : 293 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 24 kDa
Immunocytochemistry/ Immunofluorescence - Anti-CINP [EPR14445] antibody - N-terminal (ab183519)
Immunofluorescent analysis of 20°C Acetone fixed HeLa cells labeling CINP with ab183519 at 1/100 dilution (green). Secondary antibody: Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 dilution. Right panel is counterstained with Dapi (blue).
Immunoprecipitation - Anti-CINP [EPR14445] antibody - N-terminal (ab183519)
Western blot analysis of CINP in immunoprecipitation pellets from 293 lysate.
Lane 1: ab183159 used at a 1/50 dilution.
Lane 2: TBS negative control.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500.