概述

• 产品名称Anti-CKII alpha抗体[EP1963Y]
     

• 描述

  兔单克隆抗体[EP1963Y] to CKII alpha

• 经测试应用WB,Flow Cyt,IHC-P

• 种属反应性

  与反应: Mouse, Rat, Human    
   

• 免疫原

  Synthetic peptide corresponding to residues near the N terminus of human CKII alpha protein

• 阳性对照    

• WB: HeLa cell lysate IHC-P Human breast carcinoma tissue

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

   

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

• 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

• 纯度Tissue culture supernatant

• 克隆单克隆

• 克隆编号EP1963Y

• 同种型IgG

• 研究领域

• Signal Transduction

• Protein Phosphorylation

• Ser / Thr Kinases

• Other Kinases

• Cell Biology

• Apoptosis

• Intracellular

• Kinases

• <a javascript:void(0)>Cell Biology

• Cell Cycle

• Kinases/Phosphatases

• Other

Anti-CKII alpha antibody [EP1963Y] 图像

• 

  Flow Cytometry - Anti-CKII alpha antibody [EP1963Y] (ab76040)          

     Overlay histogram showing Jurkat cells stained with ab76040 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76040, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.  

• 

  Western blot - CKII alpha antibody [EP1963Y] (ab76040)          

     Anti-CKII alpha antibody [EP1963Y] (ab76040) at 1/1000 dilution + HeLa cell lysate at 10 µg
  
  Secondary
  HRP labelled goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 45 kDa
  Observed band size : 45 kDa
   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CKII alpha antibody [EP1963Y] (ab76040)          

     Immunohistochemical analysis of CKII alpha in paraffin embedded human breast carcinoma tissue using ab76040 at a 1/50 dilution.