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详细说明
概述
产品名称Anti-CKLF抗体[EPR11985(2)] - C-terminal
描述
兔单克隆抗体[EPR11985(2)] to CKLF - C-terminal
经测试应用WB,IHC-P
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CKLF aa 100 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q9UBR5阳性对照
K562, HeLa, Jurkat and THP-1 cell lysates, Paraffin-embedded Human pancreas tissue
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR11985(2)
同种型IgG
研究领域
Immunology
Innate Immunity
Macrophage / Inflamm.
Anti-CKLF antibody [EPR11985(2)] - C-terminal 图像
![Western blot - Anti-CKLF [EPR11985(2)] antibody (ab180512)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab180512wb1.jpg)
Western blot - Anti-CKLF [EPR11985(2)] antibody (ab180512)
All lanes : Anti-CKLF antibody [EPR11985(2)] - C-terminal (ab180512) at 1/5000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 17 kDa
Observed band size : 17 kDa
![Western blot - Anti-CKLF [EPR11985(2)] antibody (ab180512)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab180512wb2.jpg)
Western blot - Anti-CKLF [EPR11985(2)] antibody (ab180512)
All lanes : Anti-CKLF antibody [EPR11985(2)] - C-terminal (ab180512) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : THP-1 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 17 kDa
Observed band size : 17 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKLF [EPR11985(2)] antibody (ab180512)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab180512wb3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKLF [EPR11985(2)] antibody (ab180512)
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling CKLF with ab180512 at 1/100 dilution. Anti-rabbit HRP secondary antibody was used and counterstained with Hematoxylin.
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