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详细说明
概述
产品名称Anti-CD31 (phospho Y713)抗体[EPR8079(2)] - C-terminal
描述
兔单克隆抗体[EPR8079(2)] to CD31 (phospho Y713) - C-terminal
特异性ab180175 recognizes only the Y713-Phosphorylated form of Human CD31.
经测试应用WB,Flow Cyt,ICC/IF
种属反应性
与反应: Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CD31 aa 700 to the C-terminus (C terminal) (phospho Y713) (Cysteine residue). The exact sequence is proprietary.
Database link: P16284阳性对照
Pervanadate-treated Jurkat cells and lysate.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 49% PBS, 0.05% BSA纯度Tissue culture supernatant
克隆单克隆
克隆编号EPR8079(2)
同种型IgG
研究领域
Cardiovascular
Cardiovascular Markers
Cell Markers
Endothelial Cells
Stem Cells
Hematopoietic Progenitors
Myeloid
Thrombocytic Lineage
Stem Cells
Hematopoietic Progenitors
Myeloid
Neutrophil Lineage
Stem Cells
Hematopoietic Progenitors
Myeloid
Monocytic Lineage
Stem Cells
Hematopoietic Progenitors
Lymphoid
T Lymphocytic Lineage
Cardiovascular
Atherosclerosis
Vascular Inflammation
Leukocyte recruitment
Cell adhesion molecules
Cancer
Tumor immunology
CD markers
Cancer
Invasion/microenvironment
ECM
Cell adhesion
Other
Stem Cells
Mesenchymal Stem Cells
Surface Molecules
Cardiovascular
Angiogenesis
Angiogenic Factors
Signal Transduction
Cytoskeleton / ECM
Cell Adhesion
Cell Adhesion Molecules
Endothelial
Cardiovascular
Blood
Platelets
Anti-CD31 (phospho Y713) antibody [EPR8079(2)] - C-terminal 图像
![Western blot - Anti-CD31 (phospho Y713) [EPR8079(2)] antibody - C-terminal (ab180175)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab180175175.jpg)
Western blot - Anti-CD31 (phospho Y713) [EPR8079(2)] antibody - C-terminal (ab180175)
All lanes : Anti-CD31 (phospho Y713) antibody [EPR8079(2)] - C-terminal (ab180175) at 1/1000 dilution
Lane 1 : Non-treated Jurkat cell lysate
Lane 2 : Pervanadate-treated Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Standard HRP goat anti-rabbit at 1/2000 dilution
developed using the ECL technique
Predicted band size : 82 kDa
![Dot Blot - Anti-CD31 (phospho Y713) antibody [EPR8079(2)] - C-terminal (ab180175)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab180175Dot.jpg)
Dot Blot - Anti-CD31 (phospho Y713) antibody [EPR8079(2)] - C-terminal (ab180175)
Dot blot analysis of CD31 (pY713) peptide (Lane 1) and CD31 non-phospho peptide (Lane 2) labelling CD31 (phospho Y713) with ab180175 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
![Flow Cytometry - Anti-CD31 (phospho Y713) [EPR8079(2)] antibody - C-terminal (ab180175)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1801755fc.jpg)
Flow Cytometry - Anti-CD31 (phospho Y713) [EPR8079(2)] antibody - C-terminal (ab180175)
Flow cytometric analysis of permeabilized, Pervanadate-treated Jurkat cells labeling CD31 (Phospho Y713) using ab180175 at 1/10 (red) or a rabbit IgG negative (green).
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