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详细说明
概述
产品名称Anti-Cytochrome p450 2C19抗体[EPR6577] - C-terminal
描述
兔单克隆抗体[EPR6577] to Cytochrome p450 2C19 - C-terminal
经测试应用WB,Flow Cyt,IP,ICC/IF
种属反应性
与反应: Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) aa 450 to the C-terminus. The exact sequence is proprietary.
Database link: P33261阳性对照
HeLa cells, 293 cells, Human fetal liver tissue.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR6577
同种型IgG
研究领域
Metabolism
Pathways and Processes
Mitochondrial Metabolism
Cytochromes
Metabolism
Pathways and Processes
Metabolic signaling pathways
Drug metabolism
Metabolism
Pathways and Processes
Metabolic signaling pathways
Lipid and lipoprotein metabolism
Lipases
Signal Transduction
Metabolism
Drug metabolism
Cardiovascular
Lipids / Lipoproteins
Lipid Metabolism
Cytochromes
Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal 图像
Immunoprecipitation - (ab185213)
Immunoprecipitation analysis of 293 cell lysate immunoprecipitated for Cytochrome P450 2C19 using ab185213 at 1/50 dilution (Lane 1). A aAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution was used as secondary. Lane 2: PBS instead of 293 lysates
Immunocytochemistry/ Immunofluorescence - (ab185213)
Immunofluorescent analysis of 4% paraformaldehyde fixed 293 cells labeling Cytochrome P450 2C19 with ab185213 at 1/250 followed by Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 and counterstained with Dapi.
Flow Cytometry - (ab185213)
Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling Cytochrome P450 2C19 with ab185213 at 1/240 (red) or Rabbit monoclonal IgG (green) followed by Goat anti rabbit IgG (FITC) at 1/150.
Western blot - (ab185213)
All lanes : Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213) at 1/5000 dilution
Lane 1 : HeLa cell line lysate
Lane 2 : 293 cell line lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size : 56 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)
Western blot - (ab185213)
Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213) at 1/1000 dilution + Human fetal liver lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 56 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)
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