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详细说明
概述
产品名称Anti-Gemin 5抗体[EPR17841]
描述
兔单克隆抗体[EPR17841] to Gemin 5
经测试应用WB,ICC/IF,Flow Cyt,IP
种属反应性
与反应: Human
免疫原
Recombinant fragment within Human Gemin 5 aa 1250-1500. The exact sequence is proprietary.
Database link: Q8TEQ6Run BLAST with
Run BLAST with 
阳性对照
WB: HeLa, K562 and HEK-293 cell lysates ICC/IF: HeLa and Jurkat cells. Flow Cyt: HEK-293 cells. IP: HeLa whole cell lysate.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR17841
同种型IgG
研究领域
Epigenetics and Nuclear Signaling
DNA / RNA
RNA Processing
Splicing
Anti-Gemin 5 antibody [EPR17841] 图像
![Western blot - Anti-Gemin 5 antibody [EPR17841] (ab201691)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab201691691.jpg)
Western blot - Anti-Gemin 5 antibody [EPR17841] (ab201691)
All lanes : Anti-Gemin 5 antibody [EPR17841] (ab201691) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cells from bone marrow) cell lysate
Lane 3 : HEK-293 (Human epithelial cells from embryonic kidney) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 168 kDa
Observed band size : 168 kDa
Exposure time : 7 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
![Immunocytochemistry/ Immunofluorescence - Anti-Gemin 5 antibody [EPR17841] (ab201691)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab201691912.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Gemin 5 antibody [EPR17841] (ab201691)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Gemin 5 with ab201691 at 1/600 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
-ve control 1: ab201691 at 1/600 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.![Immunocytochemistry/ Immunofluorescence - Anti-Gemin 5 antibody [EPR17841] (ab201691)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab201691913.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Gemin 5 antibody [EPR17841] (ab201691)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling Gemin 5 with ab201691 at 1/600 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on Jurkat cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
-ve control 1: ab201691 at 1/600 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.![Flow Cytometry - Anti-Gemin 5 antibody [EPR17841] (ab201691)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab201691914.jpg)
Flow Cytometry - Anti-Gemin 5 antibody [EPR17841] (ab201691)
Flow cytometric analysis of 2% paraformaldehyde-fixed HEK-293 (Human epithelial cells from embryonic kidney) cells labeling Gemin 5 with ab201691 at 1/250 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
![Immunoprecipitation - Anti-Gemin 5 antibody [EPR17841] (ab201691)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab201691915.jpg)
Immunoprecipitation - Anti-Gemin 5 antibody [EPR17841] (ab201691)
Gemin 5 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab201691 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab201691 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/1500 dilution.
Lane 1: HeLa whole cell lysate 10 µg (Input). Lane 2: ab201691 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab201691 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 3 seconds.
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