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详细说明
概述
产品名称Anti-Geminin抗体[EPR14637]
描述
兔单克隆抗体[EPR14637] to Geminin
经测试应用WB,ICC/IF,IHC-P
种属反应性
与反应: Human
免疫原
Recombinant fragment within Human Geminin aa 100 to the C-terminus. The exact sequence is proprietary.
Database link: O75496Run BLAST with
Run BLAST with 
阳性对照
WB: Untreated HeLa, HeLa (treated with thymidine), and HeLa (treated with nocodazole) whole cell lysate. IHC-P: Human triple-negative breast cancer and Human tonsil tissue. ICC/IF: MCF-7 cells
常规说明
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR14637
同种型IgG
研究领域
Cancer
Cell cycle
Cell division
Epigenetics and Nuclear Signaling
DNA / RNA
DNA Synthesis
Other
Anti-Geminin antibody [EPR14637] 图像
![Western blot - Anti-Geminin antibody [EPR14637] (ab195047)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab195047WB1.jpg)
Western blot - Anti-Geminin antibody [EPR14637] (ab195047)
All lanes : Anti-Geminin antibody [EPR14637] (ab195047) at 1/500 dilution
Lane 1 : Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) treated with thymidine whole cell lysate
Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) treated with nocodazole whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
developed using the ECL technique
Predicted band size : 24 kDa
Exposure time : 3 minutesBlocking buffer and diluting buffer was concentration 5% NFDM /TBST
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Geminin antibody [EPR14637] (ab195047)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab195047HC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Geminin antibody [EPR14637] (ab195047)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Geminin with ab195047 at 1/500 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Nucleus staining on Human tonsil tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody and ab97051 at 1/500 as secondary antibody.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Geminin antibody [EPR14637] (ab195047)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab195047HC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Geminin antibody [EPR14637] (ab195047)
Immunohistochemical analysis of paraffin-embedded Human triple-negative breast cancer tissue labeling Geminin with ab195047 at 1/500 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Nucleus staining on Human triple-negative breast cancer tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody and ab97051 at 1/500 as secondary antibody.![Immunocytochemistry/ Immunofluorescence - Anti-Geminin antibody [EPR14637] (ab195047)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab195047IF1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Geminin antibody [EPR14637] (ab195047)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (Human breast adenocarcinoma cell line) cells labeling Geminin with ab195047 at 1/100 followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500(green). Confocal image showing nuclear staining on MCF-7 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 (red).
The negative controls are as follows:-
-ve control 1 - ab195047 at 1/100 followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500.
-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500.
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