![Anti-General Receptor for phosphoinositides 1 antibody [EP394Y] 40µl](https://yunshiji.oss-cn-shenzhen.aliyuncs.com/202407/25/uzdwfhwstsf.jpg)
![Anti-Geminin antibody [EPR14637] 10µl](https://yunshiji.oss-cn-shenzhen.aliyuncs.com/202407/25/qqcot4kvph3.jpg)
![Anti-Geminin antibody [EPR14637] 40µl](https://yunshiji.oss-cn-shenzhen.aliyuncs.com/202407/25/k3dbyh2duvc.jpg)
![Anti-Geminin antibody [EPR14637] 100µl](https://yunshiji.oss-cn-shenzhen.aliyuncs.com/202407/25/adtj25w0coo.jpg)
![Anti-Gemin 5 antibody [EPR17841] 10µl](https://yunshiji.oss-cn-shenzhen.aliyuncs.com/202407/25/gahwunf00xu.jpg)
![Anti-Gemin 5 antibody [EPR17841] 40µl](https://yunshiji.oss-cn-shenzhen.aliyuncs.com/202407/25/5goa3lfailo.jpg)
详细说明
概述
产品名称Anti-ERK1 + ERK2抗体[EPR17526]
描述
兔单克隆抗体[EPR17526] to ERK1 + ERK2
经测试应用Flow Cyt,IP,ICC/IF,WB
种属反应性
与反应: Mouse, Rat, Human
免疫原
Recombinant fragment within Human ERK1 + ERK2 aa 150 to the C-terminus. The exact sequence is proprietary. Also SwissProt ID P27361
Database link: P28482Run BLAST with
Run BLAST with 
阳性对照
WB: Human ERK1 full length recombinant protein; Human ERK2 full length recombinant protein; A431, Jurkat, HeLa, HepG2, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Human fetal brain, fetal heart and fetal kidney lysates; Mouse brain, heart, kidney and spleen lysates; Rat brain, heart, kidney and spleen lysates. ICC/IF: HeLa cells. Flow Cyt: A431 cells. IP. PC-12 whole cell extract.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Alternative versions available:
Anti-ERK1 + ERK2 antibody (Alexa Fluor® 488) [EPR17526] (ab208564)
Anti-ERK1 + ERK2 antibody (Alexa Fluor® 647) [EPR17526] (ab208881)
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR17526
同种型IgG
研究领域
Cancer
Cell Death
Apoptosis
Other
Cancer
Invasion/microenvironment
Apoptosis
Other
Cancer
Invasion/microenvironment
ECM
Cell adhesion
Other
Stem Cells
Signaling Pathways
TGF beta
Cytoplasmic
Signal Transduction
Protein Phosphorylation
Ser / Thr Kinases
MAPK Pathway
Neuroscience
Neurology process
Neurodegenerative disease
Alzheimer's disease
Tangles & Tau
Anti-ERK1 + ERK2 antibody [EPR17526] 图像
![Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab184699B-1.jpg)
Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699) at 1/10000 dilution + Recombinant Human ERK1 protein ( ab43623) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size : 43, 41 kDa
Observed band size : 44 kDa ( why is the actual band size different from the predicted?)
Recombinant full length ERK1 protein (ab43623) contains aa1-379.
Blocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab184699B-2.jpg)
Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699) at 1/10000 dilution + Recombinant Human ERK2 protein ( ab43625) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size : 43, 41 kDa
Observed band size : 42 kDa ( why is the actual band size different from the predicted?)
Recombinant full length ERK2 protein (ab43625) contains aa1-360.
Blocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab184699B-3.jpg)
Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
All lanes : Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699) at 1/50000 dilution
Lane 1 : A431 (Human epidermoid carcinoma) whole cell lysates
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates
Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
Lane 4 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysates
Lane 5 : Human fetal brain lysates
Lane 6 : Human fetal heart lysates
Lane 7 : Human fetal kidney lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size : 43, 41 kDa
Observed band size : 42,44 kDa (why is the actual band size different from the predicted?)
44kDa band represents ERK1. 42kDa band represents ERK2.
Blocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab184699B-4.jpg)
Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
All lanes : Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699) at 1/10000 dilution
Lane 1 : Human fetal brain lysates
Lane 2 : Human fetal heart lysates
Lysates/proteins at 10 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 43, 41 kDa
Observed band size : 42,44 kDa (why is the actual band size different from the predicted?)
44kDa band represents ERK1. 42kDa band represents ERK2.
Blocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab184699B-5.jpg)
Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
All lanes : Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699) at 1/10000 dilution
Lane 1 : Mouse brain lysates
Lane 2 : Mouse heart lysates
Lane 3 : Mouse kidney lysates
Lane 4 : Mouse spleen lysates
Lane 5 : Rat brain lysates
Lane 6 : Rat heart lysates
Lane 7 : Rat kidney lysates
Lane 8 : Rat spleen lysates
Lane 9 : C6 (Rat glial tumor cells) whole cell lysates
Lane 10 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
Lane 11 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lane 12 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size : 43, 41 kDa
Observed band size : kDa (why is the actual band size different from the predicted?)
44kDa band represents ERK1. 42kDa band represents ERK2.
Blocking/Dilution buffer: 5% NFDM/TBST.
![Immunocytochemistry/ Immunofluorescence - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab184699-IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling ERK1 + ERK2 with ab184699 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing both nuclear and cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab184699 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.![Flow Cytometry - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab184699-FC.jpg)
Flow Cytometry - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
Flow cytometric analysis of A431 (Human epidermoid carcinoma) cells labeling ERK1 + ERK2 with ab184699 at 1/440 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
![Immunoprecipitation - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab184699-IP.jpg)
Immunoprecipitation - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
ERK1 + ERK2 were immunoprecipitated from 1mg of PC-12 (Rat adrenal gland pheochromocytoma) whole cell extract with ab184699 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab184699 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: PC-12 whole cell extract. Lane 2: PBS instead of PC-12 whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.44kDa band represents ERK1. 42kDa band represents ERK2.
粤公网安备44196802000105号
400-6226-992