详细说明
概述
产品名称Anti-ERp72抗体[EPR14825] - C-terminal
描述
兔单克隆抗体[EPR14825] to ERp72 - C-terminal
经测试应用WB,IHC-P
种属反应性
与反应: Mouse, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ERp72 aa 550 to the C-terminus. The exact sequence is proprietary.
Database link: P13667阳性对照
HeLa, Human fetal liver, MCF7 and JAR lysates; Human endometrium and Human adenocarcinoma of colon tissues.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR14825
同种型IgG
研究领域
Signal Transduction
Protein Trafficking
Chaperones
Other Chaperones
Signal Transduction
Protein Trafficking
ER Proteins
Anti-ERp72 antibody [EPR14825] - C-terminal 图像
Western blot - Anti-ERp72 [EPR14825] antibody (ab190354)
All lanes : Anti-ERp72 antibody [EPR14825] - C-terminal (ab190354) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Human fetal liver lysate
Lane 3 : MCF7 cell lysate
Lane 4 : JAR cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 73 kDa
Observed band size : 73 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp72 [EPR14825] antibody (ab190354)
Immunohistochemical analysis of paraffin-embedded Human adenocarcinoma of colon tissue labeling ERp72 with ab190354 at 1/100 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Counterstained with Heamatoxylin. Negative control also shown.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp72 [EPR14825] antibody (ab190354)
Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling ERp72 with ab190354 at 1/100 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Counterstained with Heamatoxylin. Negative control also shown.