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详细说明
概述
产品名称Anti-CPT2抗体[EPR13626] - C-terminal
描述
兔单克隆抗体[EPR13626] to CPT2 - C-terminal
经测试应用IHC-P,WB,ICC/IF
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CPT2 aa 600 to the C-terminus. The exact sequence is proprietary.
Database link: P23786阳性对照
Human fetal liver and fetal kidney lysates; Human liver and skeletal muscle tissues; MCF7 cells, Rat kidney and liver lysates, Mouse heart and kidney lysates, Mouse kidney tissue and Rat colon tissue.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Alternative versions available:
Anti-CPT2 antibody (Alexa Fluor® 488) [EPR13626] - C-terminal (ab209461)
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR13626
同种型IgG
研究领域
Metabolism
Pathways and Processes
Redox metabolism
Fatty acid oxidation
Metabolism
Pathways and Processes
Metabolic signaling pathways
Lipid and lipoprotein metabolism
Fatty acids
Metabolism
Pathways and Processes
Metabolic signaling pathways
Lipid and lipoprotein metabolism
Lipid metabolism
Metabolism
Pathways and Processes
Mitochondrial Metabolism
Mitochondrial markers
Cancer
Cancer Metabolism
Metabolic signaling pathway
Metabolism of lipids and lipoproteins
Cardiovascular
Lipids / Lipoproteins
Fatty Acids
Metabolism
Signal Transduction
Metabolism
Lipid metabolism
Signal Transduction
Metabolism
Mitochondrial
Tags & Cell Markers
Subcellular Markers
Organelles
Mitochondria
Anti-CPT2 antibody [EPR13626] - C-terminal 图像
![Western blot - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181114WB1.jpg)
Western blot - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
All lanes : Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114) at 1/2000 dilution (purified)
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : MCF-7 (human breast carcinoma) whole cell lysate
Lane 3 : Mouse heart tissue lysate
Lane 4 : Mouse kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size : 74 kDa
Observed band size : 67 kDa (why is the actual band size different from the predicted?)
Blocking and diluting buffer 5% NFDM/TBST![Western blot - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181114WB2.jpg)
Western blot - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
All lanes : Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114) at 1/1000 dilution (purified)
Lane 1 : Rat kidney tissue lysate
Lane 2 : Rat liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size : 74 kDa
Blocking and diluting buffer 5% NFDM/TBST![Western blot - Anti-CPT2 [EPR13626] antibody - C-terminal (ab181114)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1811144WB.JPG)
Western blot - Anti-CPT2 [EPR13626] antibody - C-terminal (ab181114)
All lanes : Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114) at 1/10000 dilution (unpurified)
Lane 1 : Human fetal liver tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size : 74 kDa
Observed band size : 67 kDa (why is the actual band size different from the predicted?)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181114HC3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
Immunohistochemical analysis of paraffin embedded rat colon tissue section labelling CPT2 with purified ab181114 at dilution of 1/50. The secondary antibody used was HRP-conjugated Goat Anti-Rabbit IgG H&L (ab97051) at dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181114HC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
Immunohistochemical analysis of paraffin embedded mouse kidney tissue section labelling CPT2 with purified ab181114 at dilution of 1/50. The secondary antibody used was HRP-conjugated Goat Anti-Rabbit IgG H&L (ab97051) at dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181114HC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
Immunohistochemical analysis of paraffin embedded human liver carcinoma tissue section labelling CPT2 with purified ab181114 at dilution of 1/50. The secondary antibody used was Goat Anti-Rabbit IgG H&L (HRP) (ab97051), at a dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 [EPR13626] antibody - C-terminal (ab181114)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181114HC1.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 [EPR13626] antibody - C-terminal (ab181114)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CPT2 with unpurified ab181114 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 [EPR13626] antibody - C-terminal (ab181114)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181114HC2.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 [EPR13626] antibody - C-terminal (ab181114)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling CPT2 with unpurified ab181114 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
![Immunocytochemistry/ Immunofluorescence - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181114IF1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) cells labelling CPT2 with purified ab181114 at 1/100. Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with ab7291, a mouse anti-tubulin antibody (1/1000) using ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) as the secondary. Nuclei couterstained with DAPI (blue).
For negative control 1, rabbit primary antibody was used, followed by anti-mouse secondary antibody (ab150120). For negative control 2, mouse primary antibody (ab7291) was used followed by anti-rabbit secondary antibody (ab150077).
![Immunocytochemistry/ Immunofluorescence - Anti-CPT2 [EPR13626] antibody - C-terminal (ab181114)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181114114.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CPT2 [EPR13626] antibody - C-terminal (ab181114)
Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling CPT2 with unpurified ab181114 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi.
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