详细说明
概述
- 产品名称Anti-CDCA5抗体[EPR16331] - C-terminal
- 描述 兔单克隆抗体[EPR16331] to CDCA5 - C-terminal
- 经测试应用Flow Cyt, ICC/IF, WB, IHC-P
- 种属反应性 与反应: Human
- 免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CDCA5 aa 200 to the C-terminus. The exact sequence is proprietary.
Database link: Q96FF9 - 阳性对照
- 293, HeLa, Jurkat and SH-SY5Y cell lysates; Human stomach and human endometrium adenocarcinoma tissues; HeLa and 293 cells.
- 常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
- 存储溶液Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA - 纯度Protein A purified
- 克隆单克隆
- 克隆编号EPR16331
- 同种型IgG
- 研究领域
- Cell Biology
- Cell Cycle
- Cell Division
- Chromatid Cohesion
- Epigenetics and Nuclear Signaling
- Cell cycle
- Chromosome Structure
- Chromatid Cohesion
Anti-CDCA5 antibody [EPR16331] - C-terminal 图像
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![Immunocytochemistry/ Immunofluorescence - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab192237237.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling CDCA5 with ab192237 at 1/100 dilution (5μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Negative controls: anti-CDCA5 at 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution. -
![Flow Cytometry - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab19223737f.jpg)
Flow Cytometry - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)Flow cytometric analysis of 293 cells (paraformaldehyde-fixed, 2%) labeling CDCA5 with ab192237 at 1/100 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab19223737d.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)Immunohistochemical analysis of paraffin-embedded Human endometrium adenocarcinoma tissue labeling CDCA5 with ab192237 at 1/100 (5 µg/ml) dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab19223737c.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling CDCA5 with ab192237 at 1/100 (5 µg/ml) dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
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![Western blot - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab19223737b.jpg)
Western blot - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237) at 1/1000 dilution + SH-SY5Y cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size : 28 kDa
- Western blot - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)All lanes : Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237) at 1/10000 dilution
Lane 1 : 293 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size : 28 kDa
400-6226-992