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详细说明
概述
产品名称Anti-CDK2 (phospho T160) + CDK1 (phospho T161)抗体[EPR17621]
描述
兔单克隆抗体[EPR17621] to CDK2 (phospho T160) + CDK1 (phospho T161)
经测试应用WB,Dot Blot
种属反应性
与反应: Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Cdk2 aa 150-250 (phospho T160). The exact sequence is proprietary.
Database link: P24941常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR17621
同种型IgG
Anti-CDK2 (phospho T160) + CDK1 (phospho T161) antibody [EPR17621] 图像
![Western blot - Anti-CDK2 (phospho T160) + CDK1 (phospho T161) antibody [EPR17621] (ab183554)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab183554-wb.jpg)
Western blot - Anti-CDK2 (phospho T160) + CDK1 (phospho T161) antibody [EPR17621] (ab183554)
All lanes : Anti-CDK2 (phospho T160) + CDK1 (phospho T161) antibody [EPR17621] (ab183554) at 1/1000 dilution
Lane 1 : Untreated HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysates
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates treated with 40 ng/ml nocodazole for 24 hours
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size : 34 kDa
Observed band size : 34 kDa
Exposure time : 3 minutesBlocking and diluting buffer: 5% NFDM/TBST.
![Dot Blot - Anti-CDK2 (phospho T160) + CDK1 (phospho T161) antibody [EPR17621] (ab183554)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab183554t-1.jpg)
Dot Blot - Anti-CDK2 (phospho T160) + CDK1 (phospho T161) antibody [EPR17621] (ab183554)
Dot blot analysis of CDK2 (phospho T160) phospho peptide (Lane 1) and CDK2 non peptide (Lane 2) using ab183554 at 1/1000 dilution detecting CDK2 (phospho T160) phospho peptide only. A Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 was used as secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
![Dot Blot - Anti-CDK2 (phospho T160) + CDK1 (phospho T161) antibody [EPR17621] (ab183554)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab183554t-2.jpg)
Dot Blot - Anti-CDK2 (phospho T160) + CDK1 (phospho T161) antibody [EPR17621] (ab183554)
Dot blot analysis of CDK1 (phospho T161) phospho peptide (Lane 1) and CDK1 non peptide (Lane 2) using ab183554 at 1/1000 dilution detecting CDK1 (phospho T161) phospho peptide only. A Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 was used as secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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