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详细说明
概述
产品名称Anti-CFH + CFHL1抗体[EPR17159]
描述
兔单克隆抗体[EPR17159] to CFH + CFHL1
经测试应用WB,ICC/IF,IHC-P
种属反应性
与反应: Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CFH + CFHL1 aa 150-250. The exact sequence is proprietary.
Database link: Q03591阳性对照
WB: Human serum, plasma and fetal liver lysates. IHC-P: Human liver and placenta tissues. ICC/IF: HepG2 cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR17159
同种型IgG
Anti-CFH + CFHL1 antibody [EPR17159] 图像
![Western blot - Anti-CFH + CFHL1 antibody [EPR17159] (ab200656)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab2006566WB.jpg)
Western blot - Anti-CFH + CFHL1 antibody [EPR17159] (ab200656)
All lanes : Anti-CFH + CFHL1 antibody [EPR17159] (ab200656) at 1/10000 dilution
Lane 1 : Human serum
Lane 2 : Human plasma
Lane 3 : Human fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 38 kDa
Observed band size : 38,44,180 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Based on the sequence analysis, ab200656 has 100% homology with CFH protein.
The observed MW is consistent with what has been described in the literature PMID: 19745068.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CFH + CFHL1 antibody [EPR17159] (ab200656)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab200656HCa.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CFH + CFHL1 antibody [EPR17159] (ab200656)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CFH + CFHL1 with ab200656 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.
Cytoplasm staining on Human liver tissue is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CFH + CFHL1 antibody [EPR17159] (ab200656)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab200656HCb.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CFH + CFHL1 antibody [EPR17159] (ab200656)
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling CFH + CFHL1 with ab200656 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.
Plasma staining on Human placenta tissue is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CFH + CFHL1 antibody [EPR17159] (ab200656)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab200656HCc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CFH + CFHL1 antibody [EPR17159] (ab200656)
Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling CFH + CFHL1 with ab200656 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.
Human Lung carcinoma tissue was used as a negative control for CFH + CFHL1.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-CFH + CFHL1 antibody [EPR17159] (ab200656)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab200656656.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CFH + CFHL1 antibody [EPR17159] (ab200656)
Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling CFH + CFHL1 with ab200656 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).
Cytoplasm staining on HepG2 cell line is observed.
The nuclear counter stain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab200656 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
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