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详细说明
概述
产品名称Anti-CGBP抗体[EPR19199]
描述
兔单克隆抗体[EPR19199] to CGBP
经测试应用WB,IHC-P,ICC/IF,Flow Cyt,IP
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Mouse CGBP aa 100-200. The exact sequence is proprietary.
Database link: Q9CWW7阳性对照
WB: 293T, K562, Jurkat, MOLT-4, HeLa, RAW 264,7, PC-12, L-929, NIH/3T3, L6 and F9 whole cell lysates; Human fetal heart and fetal kidney lysates; Mouse brain, mouse heart, rat brain and rat spleen lysates. IHC-P: Mouse cerebrum and rat cerebellum tissues. ICC/IF: Hela and NIH/3T3 cells. Flow Cyt: 293T and NIH/3T3 cells. IP: HeLa whole cell lysate.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR19199
同种型IgG
研究领域
Epigenetics and Nuclear Signaling
Transcription
Co-factors
Epigenetics and Nuclear Signaling
Chromatin Remodeling
Trithorax
Anti-CGBP antibody [EPR19199] 图像
![Western blot - Anti-CGBP antibody [EPR19199] (ab198977)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab198977WBa.jpg)
Western blot - Anti-CGBP antibody [EPR19199] (ab198977)
All lanes : Anti-CGBP antibody [EPR19199] (ab198977) at 1/20000 dilution
Lane 1 : 293T (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate
Lane 5 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size : 76 kDa
Observed band size : 76 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1/2/3: 30 seconds; Lane 4: 3 minutes; Lane 5: 10 seconds.
![Western blot - Anti-CGBP antibody [EPR19199] (ab198977)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab198977WBb.jpg)
Western blot - Anti-CGBP antibody [EPR19199] (ab198977)
All lanes : Anti-CGBP antibody [EPR19199] (ab198977) at 1/2000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size : 76 kDa
Observed band size : 76 kDa
Exposure time : 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-CGBP antibody [EPR19199] (ab198977)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab198977WBc.jpg)
Western blot - Anti-CGBP antibody [EPR19199] (ab198977)
All lanes : Anti-CGBP antibody [EPR19199] (ab198977) at 1/2000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size : 76 kDa
Observed band size : 76 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 10 seconds; Lane 2/3/4: 3 minutes.
![Western blot - Anti-CGBP antibody [EPR19199] (ab198977)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab198977WBd.jpg)
Western blot - Anti-CGBP antibody [EPR19199] (ab198977)
Lanes 1 - 4 : Anti-CGBP antibody [EPR19199] (ab198977) at 1/2000 dilution
Lanes 5 - 6 : Anti-CGBP antibody [EPR19199] (ab198977) at 1/10000 dilution
Lane 1 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 3 : L-929 (Mouse connective tissue fibroblast cell line) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 5 : L6 (Rat skeletal muscle cell line) whole cell lysate
Lane 6 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size : 76 kDa
Observed band size : 76 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1/2: 10 seconds; Lane 3/4/6: 8 seconds; Lane 5: 3 minutes.
![Western blot - Anti-CGBP antibody [EPR19199] (ab198977)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab1989773-a.jpg)
Western blot - Anti-CGBP antibody [EPR19199] (ab198977)
All lanes : Anti-CGBP antibody [EPR19199] (ab198977) at 1/500 dilution
Lane 1 : WT HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : CFP1(CGBP) KO HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Secondary
Goat anti-Rabbit IgG (H+L), HRP at 1/5000 dilution
Predicted band size : 76 kDa
Observed band size : 76 kDa
Exposure time : 15 secondsBlocking buffer: 5% NFDM/TBST.
Dilution buffer: 1% BSA/TBST.
This data is from our collaborator Hengyu-Fan's lab (Life Sciences Institute Zhejiang University).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGBP antibody [EPR19199] (ab198977)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab198977HCa.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGBP antibody [EPR19199] (ab198977)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling CGBP with ab198977 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Nuclear staining on mouse cerebrum tissue is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGBP antibody [EPR19199] (ab198977)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab198977HCb.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGBP antibody [EPR19199] (ab198977)
Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling CGBP with ab198977 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Nuclear staining on rat cerebellum tissue is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-CGBP antibody [EPR19199] (ab198977)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab198977IFa.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CGBP antibody [EPR19199] (ab198977)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling CGBP with ab198977 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear staining on HeLa cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin-Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab198977 at 1/250 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-CGBP antibody [EPR19199] (ab198977)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab198977IFb.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CGBP antibody [EPR19199] (ab198977)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling CGBP with ab198977 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear staining on NIH/3T3 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected Anti-alpha Tubulin antibody - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab198977 at 1/250 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
![Flow Cytometry - Anti-CGBP antibody [EPR19199] (ab198977)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab1989777FC.jpg)
Flow Cytometry - Anti-CGBP antibody [EPR19199] (ab198977)
Flow cytometric analysis of 4% paraformaldehyde-fixed 293T (Human epithelial cell line from embryonic kidney) cells labeling CGBP with ab198977 at 1/150 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A] - Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.
![Flow Cytometry - Anti-CGBP antibody [EPR19199] (ab198977)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab198977-fc.jpg)
Flow Cytometry - Anti-CGBP antibody [EPR19199] (ab198977)
Flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 cells labeling CGBP with ab198977 at 1/150 dilution (red) compared with a Rabbit IgG,monoclonal-Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (FITC) at a dilution of 1/500 was used as the secondary antibody.
![Immunoprecipitation - Anti-CGBP antibody [EPR19199] (ab198977)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab1989777IP.jpg)
Immunoprecipitation - Anti-CGBP antibody [EPR19199] (ab198977)
CGBP was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab198977 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab198977 at 1/1000 dilution.
VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/10000 dilution.
Lane 1: HeLa whole cell lysate, 10µg (Input).
Lane 2: ab198977 IP in HeLa whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A] -Isotype Control (ab172730) instead of ab198977 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
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