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详细说明
概述
产品名称Anti-C10orf46抗体[EPR16094]
描述
兔单克隆抗体[EPR16094] to C10orf46
经测试应用WB,IHC-P,IP,ICC/IF
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human C10orf46 aa 200-300. The exact sequence is proprietary.
Database link: Q86Y37阳性对照
A375, HT1080, SW480 and Human breast cancer lysates; Human cervix squamous cell carcinoma and rat brain tissues; HeLa and A375 Cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR16094
同种型IgG
研究领域
Cell Biology
Other Antibodies
Other Antibodies
Cell Biology
Proteolysis / Ubiquitin
Proteasome / Ubiquitin
Ubiquitin E3 Enzymes
SCF Complex E3 Ligase
Anti-C10orf46 antibody [EPR16094] 图像
![Western blot - Anti-C10orf46 [EPR16094] antibody (ab190799)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab190799799.jpg)
Western blot - Anti-C10orf46 [EPR16094] antibody (ab190799)
All lanes : Anti-C10orf46 antibody [EPR16094] (ab190799) at 1/1000 dilution
Lane 1 : A375 cell lysate
Lane 2 : HT1080 cell lysate
Lane 3 : SW480 cell lysate
Lane 4 : Human breast cancer lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 41 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-C10orf46 [EPR16094] antibody (ab190799)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab190799995.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-C10orf46 [EPR16094] antibody (ab190799)
Immunofluorescent analysis of 4% paraformaldehyde-fixed A375 cells labeling C10orf46 with ab190799 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue. Negative controls also shown.
![Immunocytochemistry/ Immunofluorescence - Anti-C10orf46 [EPR16094] antibody (ab190799)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab190799994.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-C10orf46 [EPR16094] antibody (ab190799)
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling C10orf46 with ab190799 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue. Negative controls also shown.
![Immunoprecipitation - Anti-C10orf46 [EPR16094] antibody (ab190799)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab190799996.jpg)
Immunoprecipitation - Anti-C10orf46 [EPR16094] antibody (ab190799)
Western blot analysis of C10orf46 in immunoprecipitation pellets from HT1080 lysate. ab190799 used at 1/40 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10orf46 [EPR16094] antibody (ab190799)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab190799993.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10orf46 [EPR16094] antibody (ab190799)
Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling C10orf46 with ab190799 at 1/50 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin (inset: negative control).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10orf46 [EPR16094] antibody (ab190799)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab190799992.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10orf46 [EPR16094] antibody (ab190799)
Immunohistochemical analysis of paraffin-embedded Human cervix squamous cell carcinoma tissue labeling C10orf46 with ab190799 at 1/50 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin (inset: negative control).
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