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详细说明
概述
产品名称Anti-CECR5抗体[EPR14793(B)] - C-terminal
描述
兔单克隆抗体[EPR14793(B)] to CECR5 - C-terminal
经测试应用WB,ICC/IF,IP
种属反应性
与反应: Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CECR5 aa 350 to the C-terminus. The exact sequence is proprietary.
Database link: Q9BXW7阳性对照
HeLa, Jurkat, HepG2, U87-MG cell lysates.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Tissue culture supernatant
克隆单克隆
克隆编号EPR14793(B)
同种型IgG
研究领域
Cell Biology
Other Antibodies
Other Antibodies
Anti-CECR5 antibody [EPR14793(B)] - C-terminal 图像
![Western blot - Anti-CECR5 [EPR14793(B)] antibody - C-terminal (ab184185)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab184185185.jpg)
Western blot - Anti-CECR5 [EPR14793(B)] antibody - C-terminal (ab184185)
All lanes : Anti-CECR5 antibody [EPR14793(B)] - C-terminal (ab184185) at 1/5000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 46 kDa
![Western blot - Anti-CECR5 [EPR14793(B)] antibody (ab184185)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab184185WB2.jpg)
Western blot - Anti-CECR5 [EPR14793(B)] antibody (ab184185)
All lanes : Anti-CECR5 antibody [EPR14793(B)] - C-terminal (ab184185) at 1/1000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
developed using the ECL technique
Predicted band size : 46 kDa
![Immunoprecipitation - Anti-CECR5 [EPR14793(B)] antibody (ab184185)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1841855IP.jpg)
Immunoprecipitation - Anti-CECR5 [EPR14793(B)] antibody (ab184185)
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling CECR5 with ab184185. Right lane shows PBS negative control.
![Immunocytochemistry/ Immunofluorescence - Anti-CECR5 [EPR14793(B)] antibody (ab184185)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1841855IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CECR5 [EPR14793(B)] antibody (ab184185)
Immunofluorescent analysis of HeLa cells labeling CECR5 with ab184185 at a 1/100 dilution. Primary incubation was followed by secondary antibody labeling with Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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