详细说明
概述
- 产品名称Anti-Collagen VI抗体[EPR17077] - C-terminal
- 描述 兔单克隆抗体[EPR17077] to Collagen VI - C-terminal
- 经测试应用IHC-P, WB, ICC/IF
- 种属反应性 与反应: Mouse, Rat, Human
- 免疫原
Recombinant fragment within Human Collagen VI aa 800 to the C-terminus. The exact sequence is proprietary.
Database link: P12109Run BLAST with
Run BLAST with 
- 阳性对照
- WB: Human fetal heart, skeletal muscle and WI-38 (Human lung) whole cell lysate. Mouse heart, kidney and spleen. Rat heart, kidney and spleen. NIH/3T3 whole cell lysate. IHC-P: Human colon and Mouse cardiac muscle tissue. ICC/IF: NIH/3T3 cells.
- 常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
- 存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA - 纯度Protein A purified
- 克隆单克隆
- 克隆编号EPR17077
- 同种型IgG
- 研究领域
- Signal Transduction
- Cytoskeleton / ECM
- Extracellular Matrix
- ECM Proteins
- Collagen
- Cancer
- Invasion/microenvironment
- ECM
- Extracellular matrix
- Other
Anti-Collagen VI antibody [EPR17077] - C-terminal 图像
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![Western blot - Anti-Collagen VI antibody [EPR17077] (ab199720)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab199720720.JPG)
Western blot - Anti-Collagen VI antibody [EPR17077] (ab199720)All lanes : Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720) at 1/10000 dilution
Lane 1 : Human fetal heart
Lane 2 : Human skeletal muscle
Lane 3 : WI-38 (Human lung) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 109 kDa
Observed band size : 147 kDa (why is the actual band size different from the predicted?)
Exposure time : 15 secondsBlocking/dilution buffer: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the following literature: PMID:16130093 and 21186846
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![Western blot - Anti-Collagen VI antibody [EPR17077] (ab199720)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab199720202.JPG)
Western blot - Anti-Collagen VI antibody [EPR17077] (ab199720)All lanes : Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720) at 1/1000 dilution
Lane 1 : Mouse heart
Lane 2 : Mouse kidney
Lane 3 : Mouse spleen
Lane 4 : Rat heart
Lane 5 : Rat kidney
Lane 6 : Rat spleen
Lane 7 : NIH/3T3 (mouse embryo) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 109 kDa
Observed band size : 147 kDa (why is the actual band size different from the predicted?)
Exposure time : 10 secondsBlocking/dilution buffer: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the following literature: PMID:16130093 and 21186846
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17077] (ab199720)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab199720203.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17077] (ab199720)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Collagen VI with ab199720 at 1/1600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on stromal cells of Human colon tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17077] (ab199720)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab199720204.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17077] (ab199720)Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling Collagen VI with ab199720 at 1/1600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Extracellular matrix staining on mouse cardiac muscle tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).
400-6226-992