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详细说明
概述
产品名称Anti-COPE抗体[EPR12579]
描述
兔单克隆抗体[EPR12579] to COPE
经测试应用WB,IHC-P,ICC/IF,Flow Cyt
种属反应性
与反应: Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human COPE aa 50-150. The exact sequence is proprietary.
Database link: O14579阳性对照
MCF-7, PC-3, HeLa and A375 cell lysate. Human liver tissue. MCF-7 and A375 cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR12579
同种型IgG
研究领域
Signal Transduction
Protein Trafficking
Vesicle Transport
Coat Proteins
Anti-COPE antibody [EPR12579] 图像
![Western blot - Anti-COPE [EPR12579] antibody (ab180946)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab180946461.jpg)
Western blot - Anti-COPE [EPR12579] antibody (ab180946)
All lanes : Anti-COPE antibody [EPR12579] (ab180946) at 1/5000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : PC-3 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A375 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 34 kDa
Blocking/ Dilution buffer: 5% NFDM /TBST.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COPE [EPR12579] antibody (ab180946)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab180946462.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COPE [EPR12579] antibody (ab180946)
Immunohistochemical analysis of paraffin embedded Human liver tissue sections labeling COPE using ab180946 at a 1/50 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
![Immunocytochemistry/ Immunofluorescence - Anti-COPE [EPR12579] antibody (ab180946)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab180946463.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-COPE [EPR12579] antibody (ab180946)
Immunofluorescent analysis of formaldehyde fixed MCF-7 cells labeling COPE using ab180946 at a 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) was used as the secondary at a 1/200 dilution. Counterstain DAPI.
![Flow Cytometry - Anti-COPE [EPR12579] antibody (ab180946)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab180946464.jpg)
Flow Cytometry - Anti-COPE [EPR12579] antibody (ab180946)
Flow Cytometry analysis of A375 cells labeling COPE using ab180946 at a 1/70 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
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