详细说明
概述
- 产品名称Anti-GEF H1抗体[EPR17963] - C-terminal
- 描述 兔单克隆抗体[EPR17963] to GEF H1 - C-terminal
- 经测试应用ICC/IF, Flow Cyt, WB
- 种属反应性 与反应: Mouse, Rat, Human
- 免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human GEF H1 aa 950 to the C-terminus. The exact sequence is proprietary.
Database link: Q92974 - 阳性对照
- WB: HeLa, HEK293, C6 and NIH/3T3 cell lysate. ICC/IF: HeLa and MCF-7 cells. FC: HEK293 cells.
- 常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
- 存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA - 纯度Protein A purified
- 克隆单克隆
- 克隆编号EPR17963
- 同种型IgG
- 研究领域
- Signal Transduction
- Cytoskeleton / ECM
- Cytoskeleton
- Microtubules
- MT Associated Proteins
- MAP
- Cancer
- Signal transduction
- G protein signaling
- Small G proteins
- Ras family
Anti-GEF H1 antibody [EPR17963] - C-terminal 图像
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![Western blot - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab201687687.jpg)
Western blot - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687)All lanes : Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687) at 1/10000 dilution
Lane 1 : HEK293 (Human embryonic kidney) lysate
Lane 2 : HEK293 (Human epithelial cells from cervix adenocarcinoma) lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 112 kDa
Observed band size : 112 kDa
Exposure time : 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab201687872.jpg)
Western blot - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687)All lanes : Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687) at 1/2000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) lysate
Lane 4 : C6 (Rat glial tumor cells) lysate
Lane 5 : NIH/3T3 (Mouse embyro fibroblast cells)
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 112 kDa
Observed band size : 112 kDa
Exposure time : 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab201687873.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling GEF H1 with ab201687 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).
Confocal image showing cytoplasmic staining on HeLa cell line is observed.
The nuclear counter stain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. ab201687 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution. -
![Immunocytochemistry/ Immunofluorescence - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab201687874.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling GEF H1 with ab201687 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).
Confocal image showing cytoplasmic staining on MCF7 cell line is observed.
The nuclear counter stain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. ab201687 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution. -
![Flow Cytometry - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab201687875.jpg)
Flow Cytometry - Anti-GEF H1 antibody [EPR17963] - C-terminal (ab201687)Flow cytometric analysis of 2% paraformaldehyde-fixed HEK293 (human embryonic kidney) cells labeling GEF H1 with ab201687 at 1/100 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
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