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详细说明
概述
产品名称Anti-C4BPA抗体[EPR14780]
描述
兔单克隆抗体[EPR14780] to C4BPA
经测试应用WB,IHC-P
种属反应性
与反应: Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human C4BPA aa 50-150. The exact sequence is proprietary.
Database link: P04003阳性对照
WB: Human plasma lysate. IHC-P: Human transitional cell carcinoma of bladder tissue.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR14780
同种型IgG
研究领域
Immunology
Innate Immunity
Complement
Classical Pathway
Anti-C4BPA antibody [EPR14780] 图像
![Western blot - Anti-C4BPA antibody [EPR14780] (ab200345)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab200345456.jpg)
Western blot - Anti-C4BPA antibody [EPR14780] (ab200345)
Anti-C4BPA antibody [EPR14780] (ab200345) at 1/5000 dilution + human plasma lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 67 kDa
Observed band size : 67 kDa
Exposure time : 30 seconds5% NFDM/TBST: Blocking and diluting buffer.
![Western blot - Anti-C4BPA antibody [EPR14780] (ab200345)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab200345neg.jpg)
Western blot - Anti-C4BPA antibody [EPR14780] (ab200345)
All lanes : Anti-C4BPA antibody [EPR14780] (ab200345) at 1/5000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetalkidney lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 4 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 67 kDa
Observed band size : 67 kDa
Exposure time : 30 seconds5% NFDM/TBST: Blocking and diluting buffer.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4BPA antibody [EPR14780] (ab200345)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab200345455.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4BPA antibody [EPR14780] (ab200345)
Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling C4BPA using ab200345 at 1/100 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
Inset image: negative control obtained using PBS instead of ab200345 and secondary antibody only.
Note: Plasma staining on human transitional cell carcinoma of bladder tissue was observed.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4BPA antibody [EPR14780] (ab200345)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling C4BPA using ab200345 at 1/100 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
Inset image: negative control obtained using PBS instead of ab200345 and secondary antibody only.
Note: No staining on human skeletal muscle tissue was observed.
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