详细说明
概述
产品名称Anti-C6orf64抗体[EPR15780] - C-terminal
描述
兔单克隆抗体[EPR15780] to C6orf64 - C-terminal
经测试应用IHC-P,ICC/IF,WB
种属反应性
与反应: Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human C6orf64 aa 150 to the C-terminus. The exact sequence is proprietary.
Database link: Q9NPB0阳性对照
Human testis, Human fetal liver, 293T and MCF7 cell lysates; Human kidney tissue; HepG2 cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR15780
同种型IgG
研究领域
Cell Biology
Other Antibodies
Other Antibodies
Anti-C6orf64 antibody [EPR15780] - C-terminal 图像
Immunocytochemistry/ Immunofluorescence - Anti-C6orf64 antibody [EPR15780] - C-terminal (ab189918)
Immunofluorescent analysis of paraformaldehyde-fixed HepG2 cells labeling C6orf64 with ab189918 at 1/100, Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and DAPI staining (blue).
Western blot - Anti-C6orf64 [EPR15780] antibody - C-terminal (ab189918)
All lanes : Anti-C6orf64 antibody [EPR15780] - C-terminal (ab189918) at 1/10000 dilution
Lane 1 : Human testis cell lysate
Lane 2 : Human fetal liver cell lysate
Lane 3 : 293T cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 20 kDa
Additional bands at : 20 kDa. We are unsure as to the identity of these extra bands.Immunoprecipitation - Anti-C6orf64 [EPR15780] antibody - C-terminal (ab189918)
Immunoprecipitation of C6orf64 from Human fetal liver lysate using ab189918 at 1/50 or 1X PBS (negative control) (lane 2). Immunostained with ab189918 at 1/10000 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C6orf64 [EPR15780] antibody - C-terminal (ab189918)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling C6orf64 with ab189918 at 1/50 with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.