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详细说明
概述
产品名称Anti-CA12抗体[EPR14861] - C-terminal
描述
兔单克隆抗体[EPR14861] to CA12 - C-terminal
经测试应用Flow Cyt,IHC-P,WB
种属反应性
与反应: Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CA12 aa 300 to the C-terminus. The exact sequence is proprietary.
Database link: O43570阳性对照
MCF7, SKOV3, 293, HeLa, A549, Human fetal kidney, Human ovary cancer and Human breast cancer lysates; Human renal adenocarcinoma, ovarian carcinoma, bladder transitional cell carcinoma, pancreas, colon and stomach tissues; SK-OV-3 cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR14861
同种型IgG
研究领域
Cancer
Tumor biomarkers
Other
Anti-CA12 antibody [EPR14861] - C-terminal 图像
![Flow Cytometry - Anti-CA12 antibody [EPR14861] - C-terminal (ab195233)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab19523333i.jpg)
Flow Cytometry - Anti-CA12 antibody [EPR14861] - C-terminal (ab195233)
Flow cytometric analysis of SK-OV-3 cells (paraformaldehyde-fixed, 2%) labeling CA12 with ab195233 at 1/130 dilution (red) or a Rabbit monoclonal IgG (negative) (black), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. Unlabeled cells (Blue).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CA12 antibody [EPR14861] - C-terminal (ab195233)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab19523333h.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CA12 antibody [EPR14861] - C-terminal (ab195233)
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).
Note: Cell membrane and cytoplasm staining on human stomach tissue was observed.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CA12 antibody [EPR14861] - C-terminal (ab195233)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab19523333g.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CA12 antibody [EPR14861] - C-terminal (ab195233)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).
Note: Cell membrane and cytoplasm staining on human colon tissue was observed.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CA12 antibody [EPR14861] - C-terminal (ab195233)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab19523333f.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CA12 antibody [EPR14861] - C-terminal (ab195233)
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).
Note: Cell membrane and weakly cytoplasm staining on human pancreas tissue was observed.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CA12 antibody [EPR14861] - C-terminal (ab195233)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab19523333e.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CA12 antibody [EPR14861] - C-terminal (ab195233)
Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).
Note: Cell membrane and weakly cytoplasm staining on human transitional cell carcinoma of bladder tissue was observed.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CA12 antibody [EPR14861] - C-terminal (ab195233)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab19523333d.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CA12 antibody [EPR14861] - C-terminal (ab195233)
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).
Note: Cell membrane and weakly cytoplasm staining on human ovarian carcinoma tissue was observed.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CA12 antibody [EPR14861] - C-terminal (ab195233)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab19523333c.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CA12 antibody [EPR14861] - C-terminal (ab195233)
Immunohistochemical analysis of paraffin-embedded Human renal adenocarcinoma tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).
Note: Cell membrane and cytoplasm staining on human renal adenocarcinoma tissue was observed.
![Western blot - Anti-CA12 antibody [EPR14861] - C-terminal (ab195233)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab19523333b.jpg)
Western blot - Anti-CA12 antibody [EPR14861] - C-terminal (ab195233)
All lanes : Anti-CA12 antibody [EPR14861] - C-terminal (ab195233) at 1/10000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : SKOV3 cell lysate
Lane 3 : 293 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : SKOV3 cell lysate
Lane 6 : Human fetal kidney lysate
Lane 7 : Human ovary cancer lysate
Lane 8 : Human breast cancer lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
developed using the ECL technique
Predicted band size : 39 kDa
Exposure time : 10 seconds
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