![Anti-C4BPA antibody [EPR14781(B)] - C-terminal 10µl](https://yunshiji.oss-cn-shenzhen.aliyuncs.com/202407/25/z54e0xsk0gu.jpg)
![Anti-C4BPA antibody [EPR14781(B)] - C-terminal 40µl](https://yunshiji.oss-cn-shenzhen.aliyuncs.com/202407/25/hfwuaxd4v2i.jpg)
![Anti-C4BPA antibody [EPR14781(B)] - C-terminal 100µl](https://yunshiji.oss-cn-shenzhen.aliyuncs.com/202407/25/gyg2gtprx0x.jpg)
![Anti-C4BPA antibody [EPR14780] 10µl](https://yunshiji.oss-cn-shenzhen.aliyuncs.com/202407/25/jw4fjy2prg1.jpg)
![Anti-C4BPA antibody [EPR14780] 40µl](https://yunshiji.oss-cn-shenzhen.aliyuncs.com/202407/25/4udaqetsj2g.jpg)
![Anti-C4BPA antibody [EPR14780] 100µl](https://yunshiji.oss-cn-shenzhen.aliyuncs.com/202407/25/boguhruyrsa.jpg)
详细说明
概述
产品名称Anti-DNAJB6抗体[EPR17122] - N-terminal
描述
兔单克隆抗体[EPR17122] to DNAJB6 - N-terminal
经测试应用WB,Flow Cyt,ICC/IF,IHC-P
种属反应性
与反应: Mouse, Rat, Human
免疫原
Recombinant fragment within Human DNAJB6 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: O75190Run BLAST with
Run BLAST with 
阳性对照
U87-MG, SH-SY5Y, HeLa and Jurkat lysate. Mouse heart, rat brain, rat heart, C6, RAW264.7 and PC-12 lysate. Human testis tissue. Rat cerebral cortex tissue. SH-SY5Y and Jurkat cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR17122
同种型IgG
研究领域
Signal Transduction
Cytoskeleton / ECM
Cytoskeleton
Intermediate Filaments
Class I
Cytokeratins
Signal Transduction
Protein Trafficking
Chaperones
Heat Shock Proteins
Neuroscience
Neurology process
Neurodegenerative disease
Huntington's disease
Anti-DNAJB6 antibody [EPR17122] - N-terminal 图像
![Western blot - Anti-DNAJB6 antibody [EPR17122] (ab198995)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab198995951.jpg)
Western blot - Anti-DNAJB6 antibody [EPR17122] (ab198995)
All lanes : Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995) at 1/1000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 36 kDa
![Western blot - Anti-DNAJB6 antibody [EPR17122] (ab198995)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab198995952.jpg)
Western blot - Anti-DNAJB6 antibody [EPR17122] (ab198995)
Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995) at 1/10000 dilution + Jurkat cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 36 kDa
![Western blot - Anti-DNAJB6 antibody [EPR17122] (ab198995)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab198995953.jpg)
Western blot - Anti-DNAJB6 antibody [EPR17122] (ab198995)
All lanes : Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995) at 1/1000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Rat brain lysate
Lane 3 : Rat heart lysate
Lane 4 : C6 cell lysate
Lane 5 : RAW264.7 cell lysate
Lane 6 : PC-12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 36 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJB6 antibody [EPR17122] (ab198995)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab198995954.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJB6 antibody [EPR17122] (ab198995)
Immunohistochemical analysis of paraffin-embedded human testis tissue sections labeling DNAJB6 with ab198995 at a 1/250 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJB6 antibody [EPR17122] (ab198995)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab198995955.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJB6 antibody [EPR17122] (ab198995)
Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue sections labeling DNAJB6 with ab198995 at a 1/250 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.
![Immunocytochemistry/ Immunofluorescence - Anti-DNAJB6 antibody [EPR17122] (ab198995)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab198995956.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DNAJB6 antibody [EPR17122] (ab198995)
Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y cell line labeling DNAJB6 with ab198995 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized using 0.1% Triton X-100. ab150077 AlexaFluor®488 Goat anti-Rabbit was used as the secondary at a 1/500 dilution. Counterstained using ab7291 anti-Tubulin (mouse mAb) at a 1/1000 dilution with ab150120 AlexaFluor®594 Goat anti-Mouse secondary at a 1/500 dilution.
![Flow Cytometry - Anti-DNAJB6 antibody [EPR17122] (ab198995)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab198995957.jpg)
Flow Cytometry - Anti-DNAJB6 antibody [EPR17122] (ab198995)
Flow cytometry analysis of DNAJB6 expression in 2% paraformaldehyde-fixed Jurkat (Human acute T cell leukemia) cells cells using ab198995 at 1/500 dilution (red), a Rabbit monoclonal IgG (ab172730) IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody.
![Immunoprecipitation - Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab198995-IP.jpg)
Immunoprecipitation - Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995)
DNAJB6 was immunoprecipitated from Jurkat (Human acute T cell leukemia) whole cell extract with ab198995 at 1/150 dilution. Western blot was performed from the immunoprecipitate using ab198995 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Jurkat (Human acute T cell leukemia) whole cell extract (Input) 10 µg.
Lane 2: ab198995 IP in Jurkat (Human acute T cell leukemia) whole cell extract.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab198995 in Jurkat (Human acute T cell leukemia) whole cell extract.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
粤公网安备44196802000105号
400-6226-992