详细说明
概述
产品名称Anti-DNAJC7抗体[EPR13349] - N-terminal
描述
兔单克隆抗体[EPR13349] to DNAJC7 - N-terminal
经测试应用WB,IHC-P,ICC/IF,Flow Cyt,IP
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DNAJC7 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q99615阳性对照
HeLa, HepG2, 293T and Jurkat cell lysates; Human breast tissue and colonic adenocarcinoma tissue; HepG2 and HeLa cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
存储溶液pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA纯度Tissue culture supernatant
克隆单克隆
克隆编号EPR13349
同种型IgG
研究领域
Cell Biology
Other Antibodies
Other Antibodies
Anti-DNAJC7 antibody [EPR13349] - N-terminal 图像
Western blot - Anti-DNAJC7 [EPR13349] antibody - N-terminal (ab179830)
All lanes : Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 56 kDa
Immunocytochemistry/ Immunofluorescence - Anti-DNAJC7 [EPR13349] antibody - N-terminal (ab179830)
Immunofluorescent analysis of HepG2 cells labeling DNAJC7 with ab179830 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 [EPR13349] antibody - N-terminal (ab179830)
Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling DNAJC7 with ab179830 at 1/50 dilution.
Immunoprecipitation - Anti-DNAJC7 [EPR13349] antibody - N-terminal (ab179830)
Western blot analysis on immunoprecipitation pellet from (1) 293T cell lysate or (2) 1X PBS (negative control) immunoprecipitated using ab179830 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Flow Cytometry - Anti-DNAJC7 [EPR13349] antibody - N-terminal (ab179830)
Flow cytometric analysis of permeabilized HeLa cells labeling DNAJC7 with ab179830 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 [EPR13349] antibody - N-terminal (ab179830)
Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling DNAJC7 with ab179830 at 1/50 dilution.