概述

• 产品名称Anti-Factor D抗体[EPR14699]
     

• 描述

  兔单克隆抗体[EPR14699] to Factor D

• 经测试应用WB

• 种属反应性

  与反应: Human    
   

• 免疫原

  Recombinant fragment within Human Factor D aa 50-250. The exact sequence is proprietary.
  Database link: P00746

       Run BLAST with      Run BLAST with    

• 阳性对照    

• WB: Human serum; THP-1, U87-MG and Molt4 cell lysates

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol, 0.05% BSA

• 
  

• 纯度Protein A purified

• 克隆单克隆

• 克隆编号EPR14699

• 同种型IgG

• 研究领域

• Immunology

• Innate Immunity

• Complement

• Alternative Pathway

Anti-Factor D antibody [EPR14699] 图像

• 

  Western blot - Anti-Factor D antibody [EPR14699] (ab204917)          

     All lanes : Anti-Factor D antibody [EPR14699] (ab204917) at 1/2000 dilution
  
  Lane 1 : Human serum
  Lane 2 : THP-1 (Human monocytic leukemia cells) cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
  
  Predicted band size : 27 kDa
  Observed band size : 25 kDa (why is the actual band size different from the predicted?)
  
  
  Exposure time : 3 minutes    

  5% NFDM/TBST: Blocking and diluting buffer.

   

• 

  Western blot - Anti-Factor D antibody [EPR14699] (ab204917)          

     All lanes : Anti-Factor D antibody [EPR14699] (ab204917) at 1/1000 dilution
  
  Lane 1 : Human serum
  Lane 2 : THP-1 (Human monocytic leukemia cells) cell lysate
  Lane 3 : U87-MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
  Lane 4 : Molt4 (Human lymphoblastic leukemia cell line) cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
  
  Predicted band size : 27 kDa
  Observed band size : 25 kDa (why is the actual band size different from the predicted?)
  
  
  Exposure time : 3 minutes    

  5% NFDM/TBST: Blocking and diluting buffer.