详细说明
概述
产品名称Anti-FH抗体[EPR11647(2)(B)] - N-terminal
描述
兔单克隆抗体[EPR11647(2)(B)] to FH - N-terminal
经测试应用ICC/IF,WB,Flow Cyt
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human FH aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P07954阳性对照
HepG2, HeLa, 293 and A549 cells lysates; HeLa cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR11647(2)(B)
同种型IgG
研究领域
Metabolism
Pathways and Processes
Metabolic signaling pathways
Energy transfer pathways
Integration of energy
Metabolism
Pathways and Processes
Metabolic signaling pathways
Energy transfer pathways
Energy Metabolism
Cancer
Cancer Metabolism
Metabolic signaling pathway
Integration of energy metabolism
Cancer
Oncoproteins/suppressors
Tumor suppressors
Other
Signal Transduction
Metabolism
Energy Metabolism
Anti-FH antibody [EPR11647(2)(B)] - N-terminal 图像
Western blot - Anti-FH [EPR11647(2)(B)] antibody - N-terminal (ab184945)
All lanes : Anti-FH antibody [EPR11647(2)(B)] - N-terminal (ab184945) at 1/2000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293 cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size : 55 kDa
Observed band size : 49 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - Anti-FH [EPR11647(2)(B)] antibody - N-terminal (ab184945)
Immunofluorescence analysis of, -20 °C acetone-fixed, HeLa cells labeling FH with ab184945 at a 1/100 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) was used at a 1/200. In blue DAPI staining.
Flow Cytometry - Anti-FH [EPR11647(2)(B)] antibody - N-terminal (ab184945)
Flow cytometry analysis of 2% paraformaldehyde-fixed HeLa cells labeling FH with ab184945 at a 1/160 dilution (red) or negative control rabbit IgG (green). Secondary antibody goat anti-rabbit IgG (FITC) at a 1/150 dilution.