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详细说明
概述
产品名称Anti-CD163抗体[EPR14643-36] - C-terminal
描述
兔单克隆抗体[EPR14643-36] to CD163 - C-terminal
经测试应用Flow Cyt,IP,WB,IHC-P
种属反应性
与反应: Human
免疫原
Recombinant fragment within Human CD163 aa 1000 to the C-terminus (C terminal). The exact sequence is proprietary.
阳性对照
Human placenta and Human fetal liver lysates; Human liver tissue; HepG2 cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Run BLAST with 
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR14643-36
同种型IgG
研究领域
Cardiovascular
Atherosclerosis
Vascular Inflammation
Innate and adaptive immunity
Immunology
Cell Type Markers
CD
Myeloid Cells
Immunology
Innate Immunity
Macrophage / Inflamm.
Anti-CD163 antibody [EPR14643-36] - C-terminal 图像
Immunoprecipitation - (ab189915)
Immunoprecipitation. ab189915 at 1/1000 labeling CD163 in Human fetal kidney lysate immunoprecipitated using ab189915 at 1/50.
Flow Cytometry - (ab189915)
Flow Cytometrical analysis of paraformaldehyde-fixed HepG2 cells labeling CD163 with ab189915 at 1/10 compared to a negative control cell. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab189915)
Immunohistochemical analysis of paraffin embedded Human liver tissue labeling CD163 with ab189915 at 1/1000 with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.
Western blot - (ab189915)
All lanes : Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915) at 1/5000 dilution
Lane 1 : Human placenta lysate
Lane 2 : Human fetal liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
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