概述

• 产品名称Anti-GOLPH2抗体[EPR3606]
     

• 描述

  兔单克隆抗体[EPR3606] to GOLPH2

• 经测试应用Flow Cyt,ICC/IF,WB,IHC-P

• 种属反应性

  与反应: Mouse, Rat, Human    
   

• 免疫原

  Synthetic peptide corresponding to residues in Human GOLPH2.

• 阳性对照    

• LnCaP and SH-SY5Y cell lysates; Human colon tissue

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

• 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

• 纯度Tissue culture supernatant

• 克隆单克隆

• 克隆编号EPR3606

• 同种型IgG

• 研究领域

• Signal Transduction

• Protein Trafficking

• Golgi Proteins

• <a javascript:void(0)>Signal Transduction

• Protein Trafficking

• Organelle Proteins

Anti-GOLPH2 antibody [EPR3606] 图像

• 

  Flow Cytometry - Anti-GOLPH2 antibody [EPR3606] (ab109628)          

     Overlay histogram showing HeLa cells stained with ab109628 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109628, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.  

• 

  Western blot - GOLPH2 antibody [EPR3606] (ab109628)          

     All lanes : Anti-GOLPH2 antibody [EPR3606] (ab109628) at 1/1000 dilution
  
  Lane 1 : LnCaP cell lysate
  Lane 2 : SH-SY5Y cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP labelled Goat anti-Rabbit at 1/2000 dilution
  
  Predicted band size : 45 kDa
   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GOLPH2 antibody [EPR3606] (ab109628)          

     Immunohistochemical analysis of GOLPH2 in paraffin-embedded Human colon tissue using ab109628 at 1/500 dilution.  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody [EPR3606] (ab109628)      This image is courtesy of an anonymous Abreview    

     ab109628 staining GOLPH2 in Human small intestine by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  
  Tissue was fixed with paraformaldehyde and blocked with 5% milk for 30 min at 37°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/1000 in diluent) for 1 hour at 37°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.     

  See Abreview