概述

• 产品名称Anti-Glutathione Peroxidase 4抗体[EPNCIR144]
     

• 描述

  兔单克隆抗体[EPNCIR144] to Glutathione Peroxidase 4

• 经测试应用WB,IHC-P,ICC/IF

• 种属反应性

  与反应: Mouse, Rat, Human    
   

• 免疫原

  Synthetic peptide, corresponding to residues in Mouse Glutathione Peroxidase 4.

• 阳性对照    

• Human kidney tissue; Human testis, Human seminoma, LnCaP, Human fetal liver, Jurkat and HepG2 cell lysates.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

   

  This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Dolph Hatfield.

  We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

• 存储溶液pH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 40% Glycerol, 0.05% BSA, 59% PBS

• 
  

• 纯度Protein A purified

• 克隆单克隆

• 克隆编号EPNCIR144

• 同种型IgG

• 研究领域

• Metabolism

• Types of disease

• Cancer

• Metabolism

• Pathways and Processes

• Redox metabolism

• Antioxidants

• Metabolism

• Pathways and Processes

• Mitochondrial Metabolism

• Mitochondrial markers

• Cancer

• Cancer Metabolism

• Cellular metabolic process

• Signal Transduction

• Metabolism

• Mitochondrial

Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] 图像

• 

  Western blot - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066)          

     All lanes : Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066) at 1/20000 dilution (purified)
  
  Lane 1 : mouse testis lysate
  Lane 2 : rat testis lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
  
  Predicted band size : 22 kDa
  Observed band size : 17 kDa (why is the actual band size different from the predicted?)
   

• 

  Western blot - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066)          

     All lanes : Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066) at 1/20000 dilution (purified)
  
  Lane 1 : human fetal liver lysate
  Lane 2 : Jurkat lysate
  
  Secondary
  HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
  
  Predicted band size : 22 kDa
  Observed band size : 17 kDa (why is the actual band size different from the predicted?)
     

  Blocking buffer: 5% NFDM/TBST

  Dilution buffer: 5% NFDM/TBST

   

• 

  Western blot - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066)          

     Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066) at 1/5000 dilution (purified) + HepG2 cell lysate at 20 µg
  
  Secondary
  HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
  
  Predicted band size : 22 kDa
  Observed band size : 17 kDa (why is the actual band size different from the predicted?)
     

  Blocking buffer: 5% NFDM/TBST

  Dilution buffer: 5% NFDM/TBST

   

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066)          

     

  Immunofluorescence staining of HEK293 cells with purified ab125066 at a working dilution of 1/200, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab125066 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066)          

     Immunohistochemical staining of paraffin embedded human stomach with purified ab125066 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066)      This image is courtesy of an Abreview submitted by Kirk McManus    

     

  Unpurified ab125066 staining Glutathione Peroxidase 4 in the HeLa cell line from Human cervical cancer by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. ab150081 an Alexa Fluor^®488-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Nuclear staining was carried out with DAPI.

     

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066)          

     

  Unpurified ab125066, at a 1/100 dilution, staining Glutathione Peroxidase 4 in paraffin-embedded Human kidney tissue by immunohistochemistry.

   

• 

  Western blot - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066)          

     All lanes : Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066) at 1/1000 dilution (unpurified)
  
  Lane 1 : Human testis lysate
  Lane 2 : Human seminoma lysate
  Lane 3 : LnCaP cell lysate
  Lane 4 : Human fetal liver lysate
  Lane 5 : Jurkat cell lysate
  Lane 6 : HepG2 cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat anti-Rabbit HRP at 1/2000 dilution
  developed using the ECL technique
  
  Predicted band size : 22 kDa