概述

• 产品名称Anti-FOXP3 [EPR15038-69]抗体- C-terminal
     

• 描述

  兔单克隆抗体[EPR15038-69] to FOXP3 - C-terminal

• 经测试应用IHC-P

• 种属反应性

  与反应: Human    
   

• 免疫原

  Recombinant fragment within Human FOXP3 aa 250 to the C-terminus. The exact sequence is proprietary.
  Database link: Q9BZS1

       Run BLAST with      Run BLAST with    

• 阳性对照    

• Human Hodgkin lymphoma tissue.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20??C long term. Avoid freeze / thaw cycle.

• 存储溶液Preservative: 0.01% Sodium azide
  Constituents: 40% Glycerol, 59% PBS, 0.05% BSA

• 
  

• 纯度Protein A purified

• 克隆单克隆

• 克隆编号EPR15038-69

• 同种型IgG

• 研究领域

• Immunology

• Adaptive Immunity

• Regulatory T Cells

• Epigenetics and Nuclear Signaling

• Transcription

• Domain Families

• Forkhead Box

• FOXP

• Cell Biology

• Cell Cycle

• Cell Division

• Chromatid Cohesion

Anti-FOXP3 [EPR15038-69] antibody - C-terminal 图像

• 

  Western blot - Anti-FOXP3 [EPR15038-69] antibody - C-terminal (ab191416)          

     All lanes : Anti-FOXP3 [EPR15038-69] antibody - C-terminal (ab191416) at 5 µg/ml
  
  Lane 1 : HEK293T cell lysate
  Lane 2 : HEK293T cell lysate overexpressing Human FOXP3
  
  Lysates/proteins at 20 µg per lane.
  
  
  Performed under reducing conditions.    

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab10563. Antibody binding was detected using Goat anti Rabbit IR680 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 [EPR15038-69] antibody - C-terminal (ab191416)          

     

  Immunohistochemical analysis of paraffin-embedded Human Hodgkin lymphoma tissue labeling FOXP3 with ab191416 at 1/250 dilution (0.9 μg/ml) followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
  Inset: Negative control: using PBS instead of primary antibody.