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详细说明
概述
产品名称Anti-CRM1 [EPR14124]抗体- C-terminal
描述
兔单克隆抗体[EPR14124] to CRM1 - C-terminal
经测试应用IHC-P,Flow Cyt,ICC/IF,WB
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CRM1 aa 1000 to the C-terminus. The exact sequence is proprietary.
Database link: O14980阳性对照
K562, HeLa, Jurkat, Ramos, C6, NIH 3T3 and PC12 cell lysates; Human bladder transitional cell carcinoma tissue; HeLa and K562 cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR14124
同种型IgG
研究领域
Epigenetics and Nuclear Signaling
Nuclear Signaling Pathways
Nuclear Receptors
Nuclear Pore Complex
Epigenetics and Nuclear Signaling
DNA / RNA
RNA Processing
Other
Signal Transduction
Protein Trafficking
Nuclear Import / Export
Anti-CRM1 [EPR14124] antibody - C-terminal 图像
![Flow Cytometry - Anti-CRM1 [O14980] antibody - C-terminal (ab191081)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab19108181e.jpg)
Flow Cytometry - Anti-CRM1 [O14980] antibody - C-terminal (ab191081)
Flow cytometric analysis of K562 cells (paraformaldehyde-fixed, 2%) labeling CRM1 with ab191081 at 1/160 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-CRM1 [O14980] antibody - C-terminal (ab191081)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab19108181d.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CRM1 [O14980] antibody - C-terminal (ab191081)
Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed; 0.1% tritonX-100-permeabilized) labeling CRM1 with ab191081 at 1/50 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRM1 [O14980] antibody - C-terminal (ab191081)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab19108181c.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRM1 [O14980] antibody - C-terminal (ab191081)
Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling CRM1 with ab191081 at 1/50 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin (inset: negative control).
![Western blot - Anti-CRM1 [O14980] antibody - C-terminal (ab191081)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab19108181b.jpg)
Western blot - Anti-CRM1 [O14980] antibody - C-terminal (ab191081)
All lanes : Anti-CRM1 [EPR14124] antibody - C-terminal (ab191081) at 1/5000 dilution
Lane 1 : C6 cell lysate
Lane 2 : PC12 cell lysate
Lane 3 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution
Predicted band size : 123 kDa
![Western blot - Anti-CRM1 [O14980] antibody - C-terminal (ab191081)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab191081081.jpg)
Western blot - Anti-CRM1 [O14980] antibody - C-terminal (ab191081)
All lanes : Anti-CRM1 [EPR14124] antibody - C-terminal (ab191081) at 1/20000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Ramos cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution
Predicted band size : 123 kDa
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