详细说明
概述
产品名称Anti-EXOSC3抗体[EPR14840(B)] - N-terminal
描述
兔单克隆抗体[EPR14840(B)] to EXOSC3 - N-terminal
经测试应用WB,IHC-P,Flow Cyt,IP,ICC/IF
种属反应性
与反应: Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human EXOSC3 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q9NQT5阳性对照
293T, HeLa, Jurkat and Raji cell lysates; Human colon tissue; Human transitional cell carcinoma of bladder tissue; 293T and HeLa cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR14840(B)
同种型IgG
研究领域
Epigenetics and Nuclear Signaling
DNA / RNA
RNA Processing
Other
Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal 图像
Immunocytochemistry/ Immunofluorescence - Anti-EXOSC3 [EPR14840(B)] antibody (ab184961)
Immunofluorescent analysis of 4% paraformaldehyde fixed 293T cells labeling EXOSC3 with ab184961 at 1/100 dilution (red). Counterstained with Dapi (blue).
Flow Cytometry - Anti-EXOSC3 [EPR14840(B)] antibody (ab184961)
Flow cytometry analysis of 2% paraformaldehyde fixed HeLa cells labeling EXOSC3 with ab184961 at 1/100 dilution (red) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.
Western blot - Anti-EXOSC3 [EPR14840(B)] antibody (ab184961)
All lanes : Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961) at 1/10000 dilution
Lane 1 : 293T cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : Jurkat cell lysates
Lane 4 : Raji cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 30 kDa
Immunoprecipitation - Anti-EXOSC3 [EPR14840(B)] antibody (ab184961)
Immunoprecipitation analysis of 293T lysates (Lane 1) labeling EXOSC3 with ab184961 at a 1/50 dilution and a negative control PBS (Lane 2). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at a 1500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 [EPR14840(B)] antibody (ab184961)
Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling EXOSC3 with ab184961 at 1/50 dilution. Counterstained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 [EPR14840(B)] antibody (ab184961)
Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded Human colon tissue labeling EXOSC3 with ab184961 at 1/50 dilution. Counterstained with Hematoxylin.