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详细说明
概述
产品名称Anti-EXOSC3抗体[EPR14841] - C-terminal
描述
兔单克隆抗体[EPR14841] to EXOSC3 - C-terminal
经测试应用ICC/IF,IP,WB,IHC-P
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human EXOSC3 aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q9NQT5阳性对照
RAW264.7, 293T, Jurkat, HeLa, NIH 3T3, mouse kidney tissue and mouse spleen tissue lysate. Rat kidney tissue lysate. Rat spleen tissue lysate. Human ovarian carcinoma tissue.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR14841
同种型IgG
研究领域
Epigenetics and Nuclear Signaling
DNA / RNA
RNA Processing
Other
Anti-EXOSC3 antibody [EPR14841] - C-terminal 图像
![Western blot - Anti-EXOSC3 [EPR14841] antibody - C-terminal (ab190689)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab190689WB3.jpg)
Western blot - Anti-EXOSC3 [EPR14841] antibody - C-terminal (ab190689)
All lanes : Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689) at 1/1000 dilution
Lane 1 : Mouse kidney tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : Rat kidney tissue lysate
Lane 4 : Rat spleen tissue lysate
Lane 5 : RAW264.7 cell line lysate
Lane 6 : NIH 3T3 cell line lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size : 30 kDa
Observed band size : 30 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-EXOSC3 [EPR14841] antibody - C-terminal (ab190689)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab1906899IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-EXOSC3 [EPR14841] antibody - C-terminal (ab190689)
Immunofluorescent analysis of 4% paraformaldehyde fixed RAW264.7 cells labeling EXOSC3 with ab190689 at 1/250, followed by Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and counterstained with DAPI.
![Immunoprecipitation - Anti-EXOSC3 [EPR14841] antibody - C-terminal (ab190689)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab1906899IP.jpg)
Immunoprecipitation - Anti-EXOSC3 [EPR14841] antibody - C-terminal (ab190689)
Lane 1: Primary: ab190689, 1/1000 dilution Sample: Lysate of 293T cells immunoprecipated with ab190689 at 1/50 Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, 1/1500 dilution.
Lane 2: Primary: ab190689, 1/1000 dilution. Sample: PBS. Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, 1/1500 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 [EPR14841] antibody - C-terminal (ab190689)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab190689HC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 [EPR14841] antibody - C-terminal (ab190689)
Immunohistochemical analysis of paraffin embedded mouse kidney tissue labeling EXOSC3 with ab190689 at 1/100, followed by prediluted, HRP conjugated secondary antibody and counterstained with hematoxylin.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 [EPR14841] antibody - C-terminal (ab190689)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab190689HC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 [EPR14841] antibody - C-terminal (ab190689)
Immunohistochemical analysis of paraffin embedded human ovarian carcinoma tissue labeling EXOSC3 with ab190689 at 1/100, followed by prediluted, HRP conjugated secondary antibody and counterstained with hematoxylin.
![Western blot - Anti-EXOSC3 [EPR14841] antibody - C-terminal (ab190689)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab190689WB2.jpg)
Western blot - Anti-EXOSC3 [EPR14841] antibody - C-terminal (ab190689)
All lanes : Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689) at 1/1000 dilution
Lane 1 : Jurkat cell line lysate
Lane 2 : HeLa cell line lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size : 30 kDa
Observed band size : 30 kDa
![Western blot - Anti-EXOSC3 [EPR14841] antibody - C-terminal (ab190689)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab190689WB1.jpg)
Western blot - Anti-EXOSC3 [EPR14841] antibody - C-terminal (ab190689)
Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689) at 1/1000 dilution + 293T cell line lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size : 30 kDa
Observed band size : 30 kDa
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