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详细说明
概述
产品名称Anti-ECH1抗体[EPR15449(B)] - C-terminal
描述
兔单克隆抗体[EPR15449(B)] to ECH1 - C-terminal
经测试应用WB,IHC-P,ICC/IF,IP,Flow Cyt
种属反应性
与反应: Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) aa 300 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q13011阳性对照
Human fetal liver, A549, Jurkat and HeLa lysates; Human endometrium and kidney tissues; MCF-7 cells; Jurkat cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR15449(B)
同种型IgG
研究领域
Metabolism
Pathways and Processes
Redox metabolism
Fatty acid oxidation
Metabolism
Pathways and Processes
Metabolic signaling pathways
Lipid and lipoprotein metabolism
Fatty acids
Metabolism
Pathways and Processes
Mitochondrial Metabolism
Mitochondrial markers
Cardiovascular
Lipids / Lipoproteins
Fatty Acids
Metabolism
Signal Transduction
Metabolism
Lipid metabolism
Tags & Cell Markers
Subcellular Markers
Organelles
Peroxisome
Tags & Cell Markers
Subcellular Markers
Organelles
Mitochondria
Anti-ECH1 antibody [EPR15449(B)] - C-terminal 图像
Western blot - (ab189255)
All lanes : Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255) at 1/50000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : A549 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size : 36 kDa
Observed band size : 33 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab189255)
Immunhistochemical analysis of paraffin-embedded human endometrium tissue labeling ECH1 with ab189254 at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab189255)
Immunhistochemical analysis of paraffin-embedded human kidney tissue labeling ECH1 with ab189254 at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Immunocytochemistry/ Immunofluorescence - (ab189255)
Immunfluorescent analysis of 100% methanol(-20℃)-fixed MCF-7 cells labeling ECH1 with ab189254 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with DAPI.
Flow Cytometry - (ab189255)
Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling ECH1 with ab189255 at 1/20 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Immunoprecipitation - (ab189255)
Western blot analysis of ECH1 in human fetal liver lysate immunoprecipitated using ab189254 at 1/50 dilution.
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
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